Six identical tRNATrpCCA genes express a similar amount of mature tRNATrpCCA but unequally contribute to yeast cell growth
Six identical tRNATrpCCA genes express a similar amount of mature tRNATrpCCA but unequally contribute to yeast cell growth
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六个相同的 tRNATrpCCA 基因表达相似量的成熟 tRNATrpCCA，但对酵母细胞生长的贡献不同
DOI：
10.1093/bbb/zbac134
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Tohru Yoshihisa
中科院分区：
文献类型：
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作者：
Sachiko Hayashi;Masaya Matsui;Ayano Ikeda;Tohru Yoshihisa
酿酒酵母有6个编码相同序列的同义tRNATrpCCA基因，包括它们的内含子区域。它们应该以相同的质量和数量表达tRNATrpCCA。在这里，我们用同义tRNATrpCCA基因的所有可能组合产生了单个到五个缺失菌株，以分析这些单独的基因是否同样有助于细胞活力和tRNA的产生。只有W(CCA)J、TW(CCA)M或TW(CCA)P的五重缺失菌株是存活的，但几乎是致命的，而其他五重缺失菌株表现出中等程度的生长障碍。这些生长差异在四重缺失菌株之间并不明显，它们在野生型中表达了近三分之一的成熟tRNATrpCCA。因此，tRNATrpCCA数量没有剂量补偿作用，五重缺失菌株之间的生长差异可能不仅仅反映了tRNATrpCCA短缺的差异。酵母可能保留tRNATrpCCA基因的冗余，以实现超出tRNA提供给翻译的非规范功能(S)。
Saccharomyces cerevisiaehas 6 synonymous tRNATrpCCAgenes encoding the identical sequence, including their intronic region. They are supposed to express tRNATrpCCAin the same quality and quantity. Here, we generated single to quintuple deletion strains with all the possible combinations of the synonymous tRNATrpCCAgenes to analyze whether those individual genes equally contribute cell viability and tRNA production. The quintuple deletion strains that only harbortW(CCA)J, tW(CCA)M, ortW(CCA)Pwere viable but almost lethal while the other quintuple deletions showed moderately impaired growth. These growth differences were not obvious among the quadruple deletion strains, which expressed almost one third of mature tRNATrpCCAin the wild type. Therefore, no dosage compensation operates for tRNATrpCCAamount, and growth variations among the quintuple deletion strains may not simply reflect differences in tRNATrpCCAshortage. Yeast may retain the redundancy of tRNATrpCCAgenes for a noncanonical function(s) beyond the supply of the tRNA to translation.