An 87-kilodalton glucan-binding protein of Streptococcus sobrinus B13.
An 87-kilodalton glucan-binding protein of Streptococcus sobrinus B13.
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远缘链球菌 B13 的 87 千道尔顿葡聚糖结合蛋白。
DOI：
10.1128/iai.60.12.5291-5293.1992
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发表时间：
1992
影响因子：
3.1
通讯作者：
Gill,RE
中科院分区：
文献类型：
--
作者：
Wu-Yuan,CD;Gill,RE
采用Sephadex G-50亲和层析和盐酸胍梯度洗脱技术，从远缘链球菌B13（d型）胞外培养上清液中分离纯化出一种分子量为87 kDa的葡聚糖结合蛋白（GBP）。蛋白质印迹（免疫印迹）分析表明，它是抗原相关的，但不完全相同，变形链球菌Ingbritt的74 kDa的GBP。87-kDa GBP没有葡萄糖基转移酶活性。GBP在S.建议使用sobrinus B13。
An 87-kDa glucan-binding protein (GBP) of Streptococcus sobrinus B13 (serotype d) was isolated and purified from extracellular culture supernatant by using affinity chromatography on Sephadex G-50 and elution with a guanidine HCl gradient. Western blot (immunoblot) analysis showed it to be antigenically related, but not completely identical, to the 74-kDa GBP of Streptococcus mutans Ingbritt. The 87-kDa GBP has no glucosyltransferase activity. A possible role for this GBP in the cariogenicity of S. sobrinus B13 is suggested.