Brilliant Pitaya-Type Silica Colloids with Central-Radial and High-Density Quantum Dots Incorporation for Ultrasensitive Fluorescence Immunoassays
Brilliant Pitaya-Type Silica Colloids with Central-Radial and High-Density Quantum Dots Incorporation for Ultrasensitive Fluorescence Immunoassays
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具有中心径向和高密度量子点掺入的明亮火龙果型二氧化硅胶体，用于超灵敏荧光免疫分析
DOI：
10.1002/adfm.201705380
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发表时间：
2018-01-24
影响因子：
19
通讯作者：
Hu, Jun
中科院分区：
文献类型：
--
作者：
Huang, Liang;Liao, Tao;Hu, Jun
从基本构建块创建二级纳米结构，同时具有高负载能力和良好控制的尺寸/均匀性，对于纳米级协同和功能单元的集成是非常需要的。这里报道了一种用多孔模板组装疏水性量子点（QD）的新策略，以形成火龙果型荧光二氧化硅胶体，在整个二氧化硅基质中具有密集且完整的量子点。具有高度可接近的中心径向孔和金属亲和内表面的巯基封端的树枝状二氧化硅球被用作强大的吸收剂主体，用于从具有高负载能力的有机相中直接固定量子点。烷基硅烷介导的二氧化硅封装可防止配体交换引起的量子点光学降解，并有利于均匀二氧化硅壳的形成。这些多重量子点嵌入二氧化硅球对不同颜色的量子点表现出良好的兼容性，具有保存完好的荧光、高胶体/光学稳定性和多种表面功能。事实证明，与侧流试纸条平台集成后，这些二氧化硅胶体可以作为有前景的荧光报告基因，为临床样品中的 C 反应蛋白提供超灵敏、特异且稳定的免疫分析。
Creating secondary nanostructures from fundamental building blocks with simultaneous high loading capacity and well-controlled size/uniformity, is highly desired for nanoscale synergism and integration of functional units. Here a novel strategy is reported for hydrophobic quantum dots (QDs) assembley with porous templates, to form pitaya-type fluorescent silica colloids with densely packed and intact QDs throughout the silica matrix. The mercapto-terminated dendritic silica spheres with highly accessible centralradial pores and metal-affinity interior surface, are adopted as a powerful absorbent host for direct immobilization of QDs from organic phase with high loading capacity. The alkylsilane mediated silica encapsulation prevents QDs' optical degradation induced by ligand exchange and favors the homogeneous silica shell formation. These multiple QD embedded silica spheres exhibit good compatibility for different colored QDs with well-preserved fluorescence, high colloidal/optical stability, and versatile surface functionality. It is demonstrated that after integration with a lateral flow strip platform, these silica colloids provide an ultrasensitive, specific, and robust immunoassay for C-reaction protein in clinical samples as promising fluorescent reporters.