Establishment of a human T-cell hybrid line with suppressive activity
Establishment of a human T-cell hybrid line with suppressive activity
复制标题
具有抑制活性的人类T细胞杂交系的建立
作者：
C. Grillot‐Courvalin;J. Brouet;R. Berger;A. Bernheim
小鼠B杂交瘤的构建可以产生几乎无限量的同质特异性抗体，这在免疫学领域取得了重大进展。最近，已经建立了分泌特定特异性抗体的人B杂交系2,3。同样，已经获得的小鼠T杂交体可能产生特异性免疫调节因子，如抗原特异性抑制因子4 - 6、T细胞替代因子7或同种异体效应因子8。人类功能性T杂交体的可用性应该能更好地理解人类淋巴细胞的相互作用和调节。我们在这里描述了一个永久的人T细胞（KE37）与外周血T淋巴细胞融合产生一个人T杂交瘤系，该系具有稳定的亚四倍体核型，并持续产生抑制美洲商陆有丝分裂原（PWM）诱导的b细胞分化的因子。
The construction of murine B hybridomas producing homogeneous specific antibodies in almost unlimited quantities has led to major progress in immunology1. Recently, human B hybrid lines secreting antibody of defined specificities have also been established2,3. Similarly, murine T hybrids have been obtained which may produce specific immunoregulatory factors such as antigen-specific suppressor factors4–6, T-cell replacing factor7 or allogeneic effect factor8. The availability of functional T hybrids in man should allow a better understanding of human lymphocyte interaction and regulation. We describe here the fusion of a permanent human T-cell (KE37) with peripheral blood T lymphocytes to produce a human T hybridoma line which has a stable hypotetraploid karyotype and continuously produces a factor which suppresses pokeweed mitogen (PWM)-induced B-cell differentiation.