EMBRYONIC CHICK CARTILAGE COLLAGENS - DIFFERENCES IN THE LOW-MR SPECIES PRESENT IN STERNAL CARTILAGE AND TIBIOTARSAL ARTICULAR-CARTILAGE
EMBRYONIC CHICK CARTILAGE COLLAGENS - DIFFERENCES IN THE LOW-MR SPECIES PRESENT IN STERNAL CARTILAGE AND TIBIOTARSAL ARTICULAR-CARTILAGE
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DOI：
10.1016/0014-5793(84)80314-2
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发表时间：
1984-01-01
期刊：
影响因子：
3.5
通讯作者：
GRANT, ME
中科院分区：
文献类型：
--
作者：
KIELTY, CM;HULMES, DJS;GRANT, ME
鸡胚胸骨和胫跗软骨中存在的胶原多肽已通过胃蛋白酶消化溶解，并通过盐分级分离。从两种组织中提取II型胶原、1α2α3α胶原和两种多肽（表观分子量150和42 kDa），它们可还原为许多较小的肽。然而，也存在于胫跗软骨的消化道炎是一个主要的不可还原的高度可溶性多肽的45 kDa。这种短链胶原蛋白显然是相同的胃蛋白酶消化产品的G胶原蛋白（Mr 59 000），一个主要的低Mrprocollagen-样种以前检测到鸡软骨细胞培养。
The collagenous polypeptides present in embryonic chick sternal and tibiotarsal cartilages have been solubilised by digestion with pepsin and separated by salt fractionation. Type II collagen, 1α2α3α collagen, and two polypeptides (apparent molecular mass 150 and 42 kDa), which were reducible to a number of smaller peptides, were extracted from both tissues. However, also present in the peptic digests of tibiotarsal cartilages was a major non-reducible highly-soluble polypeptide of 45 kDa. This short-chain collagen is apparently identical to the pepsinized product of G collagen (Mr59 000), a major low-Mrprocollagen-like species previously detected in chick chondrocyte cultures.