A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts
A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts
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DOI：
10.1016/j.jpba.2013.06.025
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发表时间：
2013-10-01
影响因子：
3.4
通讯作者：
Lapkin, Alexei A.
中科院分区：
文献类型：
--
作者：
Suberu, John;Song, Lijiang;Lapkin, Alexei A.
建立了青蒿提取物中青蒿素、9-表青蒿素、青蒿素烯、双氢青蒿酸、青蒿酸和青蒿素B的高效液相色谱-串联质谱快速测定方法。首次报道了9-表青蒿素粗提物的检测和定量。在该方法中，所有6种代谢物均在6分钟内分离并洗脱，样品制备量最少。所有分析的代谢物的回收率在96.25%和103.59%之间，青蒿素、9-表青蒿素、青蒿素和青蒿素B的标准曲线在0.15-10 μ g mL（-1）的浓度范围内呈线性（r（2）> 0.99），双氢青蒿酸和青蒿酸的标准曲线在3.75-120 μ g mL（-1）的浓度范围内呈线性（r（2）> 0.99）。所有验证指标均令人满意，表明该方法具有耐用性，快速性，灵敏度和适用于一系列应用，包括高通量（HTP）分析。(C)2013爱思唯尔有限公司版权所有。
A rapid high-pressure liquid chromatography (HPLC) tandem mass spectrometry (TQD) method for the determination of artemisinin, 9-epi-artemisinin, artemisitene, dihydroartemisinic acid, artemisinic acid and arteannuin B in Artemisia annua extracts is described. Detection and quantification of 9-epi-artemisinin in crude extracts are reported for the first time. In this method all six metabolites are resolved and eluted within 6 min with minimal sample preparation. A recovery of between 96.25% and 103.59% was obtained for all metabolites analysed and the standard curves were linear (r(2) > 0.99) over the concentration range of 0.15-10 mu g mL(-1) for artemisinin, 9-epi-artemisinin, artemisitene and arteannuin B, and the range of 3.75-120 mu g mL(-1) for dihydroartemisinic acid and artemisinic acid. All validation indices were satisfactory, showing the method to be robust, quick, sensitive and adequate for a range of applications including high throughput (HTP) analysis. (C) 2013 Elsevier B.V. All rights reserved.