Membrane transplantation to correct integral membrane protein defects.
Membrane transplantation to correct integral membrane protein defects.
复制标题
膜移植以纠正整体膜蛋白缺陷。
DOI：
10.1007/s00109-003-0446-7
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Sakalian,Michael
中科院分区：
文献类型：
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作者：
Curlee,KimberlyV;Hong,JeongS;Clancy,JP;King,ScottA;Hunter,Eric;Berdiev,Bakhrom;Benos,Dale;Sommerfelt,MajaA;Sorscher,EricJ;Sakalian,Michael
In this report we show that the tendency of certain viruses to carry host membrane proteins in their envelopes can be harnessed for transplantation of small patches of plasma membrane, including fully functional, polytopic ion channel proteins and their regulatory binding partners. As a stringent model we tested the topologically complex epithelial ion channel CFTR. Initially an attenuated vaccinia virus was found capable of transferring CFTR in a properly folded, functional and regulatable form to CFTR negative cells. Next we generated viruslike particles (VLPs) composed of retroviral structural proteins that assemble and bud at the host cell plasma membrane. These particles were also shown to mediate functional ion channel transfer. By testing the capacity of complex membrane proteins to incorporate into viral envelopes these experiments provide new insight into the permissiveness of viral envelopment, including the ability of incorporated proteins to retain function and repair defects at the cell surface, and serve as a platform for studies of ion channel and membrane protein biochemistry.
