The soybean transcription factor GmNAC085 enhances drought tolerance in Arabidopsis
The soybean transcription factor GmNAC085 enhances drought tolerance in Arabidopsis
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DOI：
10.1016/j.envexpbot.2018.03.017
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发表时间：
2018-07-01
影响因子：
5.7
通讯作者：
Lam-Son Phan Tran
中科院分区：
文献类型：
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作者：
Kien Huu Nguyen;Mostofa, Mohammad Golam;Lam-Son Phan Tran
植物特异性NAC（NAM、ATAF 1/2和CUC 2）转录因子参与调节植物对干旱的响应和耐受性。本研究利用异源拟南芥系统分析了从大豆中分离的脱水诱导型GmNAC 085在调节植物适应干旱中的功能。GmNAC 085在酵母试验中显示出作为转录激活因子，其激活结构域位于其C-末端。与野生型相比，组成型过表达GmNAC 085的转基因拟南芥（35 S：GmNAC 085）表现出轻微的生长迟缓，但提高了对干旱的耐受性，这与增强细胞膜完整性和降低水分亏缺下的蒸腾速率呈正相关。此外，降低丙二醛（脂质过氧化产物）的含量，伴随着增加的超氧化物歧化酶，过氧化氢酶和抗坏血酸过氧化物酶的活性在35 S：GmNAC 085植物表明，GmNAC 085的过表达增强抗氧化能力，以减少干旱诱导的氧化损伤的转基因植物。一致的是，一个更大的抗氧化酶编码和其他众所周知的NAC介导的干旱响应基因的表达在35 S：GmNAC 085比野生型植物在干旱下观察。这些结果揭示了GmNAC 085在调节植物干旱适应方面的积极作用，从而表明GmNAC 085在开发耐旱转基因作物方面具有潜在的应用价值。
The plant-specific NAC (NAM, ATAF1/2, and CUC2) transcription factors have been known to be involved in regulation of plant response and tolerance to drought. In this study, we analyzed the functions of the dehydration-inducible GmNAC085 isolated from soybean in regulation of plant adaptation to drought using the heterologous Arabidopsis system. GmNAC085 was shown to act as a transcriptional activator in a yeast assay, with the activation domain located in its C-terminus. In comparison with wild-type, transgenic Arabidopsis plants (35S:GmNAC085) constitutively overexpressing GmNAC085 exhibited slightly retarded growth, but improved tolerance to drought which was positively correlated with enhanced cell membrane integrity and reduced transpiration rate under water deficit. Furthermore, reduced malondialdehyde (a product of lipid peroxidation) content accompanied by increased activities of superoxide dismutase, catalase and ascorbate peroxidase in 35S:GmNAC085 plants indicated that the overexpression of GmNAC085 enhanced antioxidant capacity in the transgenic plants to reduce drought-induced oxidative damage. Consistently, a greater expression of the antioxidant enzyme-encoding and other well-known NAC-mediated drought-responsive genes was observed in 35S:GmNAC085 than in wild-type plants under drought. These results provide an insight into the positive role of GmNAC085 in regulating plant adaptation to drought, thereby suggesting potential applications of GmNAC085 for developing drought-tolerant transgenic crops.