Manometric Determination of Low Concentrations of Ethylene with Particular Reference to Plant Material
Manometric Determination of Low Concentrations of Ethylene with Particular Reference to Plant Material
复制标题
低浓度乙烯的压力测定，特别是植物材料
DOI：
10.1021/ac60063a037
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发表时间：
1952
期刊：
影响因子：
--
通讯作者：
J. B. Biale
中科院分区：
文献类型：
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作者：
R. Young;H. Pratt;J. B. Biale
微量的乙烯气体在植物代谢中起着重要作用，特别是在果实的成熟过程中。长期以来一直需要一种灵敏的定量分析方法。本文报道的方法是用高氯酸汞溶液吸收空气中的乙烯，形成乙烯-汞络合物。随后，通过加入盐酸从复合物中释放出饱和乙烯，并通过压力计测量其体积。这种方法最适合乙烯浓度为0.5 ppm或更高的情况，但不超过0.05 ppm的乙烯会被吸收。乙烯总量低至0.2 ml。可以以5%的准确度测定，而更大的量可以获得更高的准确度。尽管该方法仅对烯烃是特异性的，但已知没有除乙烯之外的气态烯烃由工厂生产。对植物代谢的一些其他挥发性产物进行了测试，没有干扰。
Ethylene gas in minute quantities plays an impor-tant role in plant metabolism, especially in the ripening of fruits. A sensitive quantitative analyti-cal method has long been needed. In the method reported here, ethylene is absorbed from an air stream in a solution of mercuric perchlorate, form-ing an ethylene-mercury complex. The accumu-lated ethylene is later released from the com-plex by the additionof hydrochloric acid, andits volume is measured manometrically. This method is best suited for ethylene concentrations of 0.5 ppm and higher, though not more than 0.05 ppm will escape absorption. Total amounts of ethylene as low as 0.2 ml. can be determined with an accuracy of 5%, while a higher accuracy can be obtained with larger amounts. Although the method is specific for olefins only, no gaseous olefin other than ethylene is known to be produced by plants. A number of other volatile products of plant metabolism were tested and did not interfere.