ADDITION OF PURIFIED OROSOMUCOID PRESERVES THE GLOMERULAR-PERMEABILITY FOR ALBUMIN IN ISOLATED PERFUSED RAT KIDNEYS
ADDITION OF PURIFIED OROSOMUCOID PRESERVES THE GLOMERULAR-PERMEABILITY FOR ALBUMIN IN ISOLATED PERFUSED RAT KIDNEYS
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DOI：
10.1111/j.1748-1716.1993.tb09466.x
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
HARALDSSON, B
中科院分区：
文献类型：
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作者：
JOHNSSON, E;HARALDSSON, B
血清蛋白，orosomucoid已被证明是维持正常的毛细血管通透性在几个不同的器官，包括肾脏。因此，在先前对离体大鼠肾脏进行的研究中，发现在不存在类乳清蛋白的情况下，白蛋白的清除率几乎高出5倍，所述离体大鼠肾脏灌注有两种市售的人白蛋白溶液中的任一种，所述两种人白蛋白溶液具有相似的组成，但类乳清蛋白的含量不同（0.21 g l-1对< 0.005 g l-1）。进行以下实验以验证对肾小球选择透过性的这种影响是由于类粘蛋白本身而不是两种溶液中的其他成分的假设。12只大鼠的两个肾脏被分离和灌注相同的白蛋白溶液中没有orosomucoid，但除了纯化orosomucoid（0.25克L-1）的肾脏之一。两组肾脏之间的血管阻力、尿流量或肾小球滤过率（GFR）无显著差异，GFR为27 +/- 2 ml min-1 100 g-1。两个肾脏的白蛋白清除分数（θ）最初相似（0.0022 +/- 0.0002）。在不存在类乳清蛋白的情况下，在灌注1小时后，θ逐渐增加至0.0076 +/- 0.0013，相比之下，在灌注液中加入类乳清蛋白的肾脏为0.0040 +/- 0.0006（P < 0.001，n = 12）。我们的结论是，血浆糖蛋白orosomucoid确实发挥了重要作用，在调节肾小球毛细血管壁的动态特性，通过减少对大分子，如白蛋白的渗透性。
The serum protein, orosomucoid has been shown to be essential for the maintenance of normal capillary permeability in several different organs, including the kidney. Thus, the clearance of albumin was found to be almost fivefold higher in the absence of orosomucoid in a previous study on isolated rat kidneys, perfused with either of two commercially available human albumin solutions of similar composition, but differing in their content of orosomucoid (0.21 g l-1 vs. < 0.005 g l-1). The following experiments were performed in order to verify the hypothesis that this effect on glomerular permselectivity was due to orosomucoid per se and not to other ingredients in the two solutions. Both kidneys of 12 rats were isolated and perfused with identical albumin solutions without orosomucoid, but with the addition of purified orosomucoid (0.25 g l-1) to one of the kidneys. No significant differences in vascular resistance, urine flow or glomerular filtration rate (GFR), which was found to be 27 +/- 2 ml min-1 100 g-1, were observed between the two groups of kidneys. The fractional clearance of albumin (theta) was initially similar for both kidneys (0.0022 +/- 0.0002). In the absence of orosomucoid, theta gradually increased to 0.0076 +/- 0.0013 after 1 h of perfusion compared to 0.0040 +/- 0.0006 for the kidneys with orosomucoid added to the perfusate (P < 0.001, n = 12). We conclude that the plasma glycoprotein orosomucoid indeed plays an important role in regulating the dynamic properties of the glomerular capillary wall by reducing the permeability towards macromolecules such as albumin.