Short Report: Leishmania major, the Predominant Leishmania Species Responsible for Cutaneous Leishmaniasis in Mali
Short Report: Leishmania major, the Predominant Leishmania Species Responsible for Cutaneous Leishmaniasis in Mali
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DOI：
10.4269/ajtmh.12-0434
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发表时间：
2013-03-01
影响因子：
3.3
通讯作者：
Doumbia, Seydou
中科院分区：
文献类型：
--
作者：
Paz, Carlos;Samake, Sibiry;Doumbia, Seydou
大利什曼原虫是已知在马里引起皮肤利什曼病的唯一利什曼原虫。我们扩增了保存在马里巴马科国家疟疾防治中心（CNAM）临床疑似CL的自我转诊患者的吉姆萨染色皮肤刮玻片上的利什曼原虫DNA，以确定马里是否有其他利什曼原虫物种导致CL，并评估其地理分布。采用利什曼原虫物种特异性引物对进行聚合酶链反应（PCR）扩增，该引物对可以扩增马里可能引起CL的主要L.、热带L.、婴儿L.和多诺瓦尼L.寄生虫的DNA。在马里5个地区（Kayes、Koulikoro、Segou、Mopti和Tombouctou）的41例显微镜下确诊的CL病例中，唯一检出的菌种是L. major。这些结果表明，主要L. major是主要的，可能是唯一的物种负责CL在马里。
Leishmania major is the only species of Leishmania known to cause cutaneous leishmanisis (CL) in Mali. We amplified Leishmania DNA stored on archived Giemsa-stained dermal scraping slides obtained from self-referral patients with clinically suspected CL seen in the Center National d'Appui A La Lutte Contre La Maladie (CNAM) in Bamako, Mali, to determine if any other Leishmania species were responsible for CL in Mali and evaluate its geographic distribution. Polymerase chain reaction (PCR) amplification was performed using a Leishmania species-specific primer pair that can amplify DNA from L. major, L. tropica, L. infantum, and L. donovani parasites, possible causative agents of CL in Mali. L. major was the only species detected in 41 microscopically confirmed cases of CL from five regions of Mali (Kayes, Koulikoro, Segou, Mopti, and Tombouctou). These results implicate L. major as the predominant, possibly exclusive species responsible for CL in Mali.