High-density linkage mapping and distribution of segregation distortion regions in the oak genome.
High-density linkage mapping and distribution of segregation distortion regions in the oak genome.
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DOI：
10.1093/dnares/dsw001
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Plomion C
中科院分区：
文献类型：
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作者：
Bodénès C;Chancerel E;Ehrenmann F;Kremer A;Plomion C
我们开发了一个基于单核苷酸多态性（SNP）的连锁遗传图谱。基于8 k基因的SNP阵列被用来对来自两个种内和两个种间全同胞家系的1,000多个全同胞进行基因分型。两个物种的8个亲本图谱之间存在高度的共线性。然后，在橡树基因组的12个连锁群（LGs）上建立了一个复合图谱，其中有4，261个SNP标记，跨度为742 cM。来自六个LG的九个基因组区域显示出高度显著的分离扭曲。两个主要的假设有关的机制分离失真进行了讨论：遗传负荷与生殖障碍。我们的研究结果表明，占主导地位的前合子后合子障碍。
We developed the densest single-nucleotide polymorphism (SNP)-based linkage genetic map to date for the genus Quercus. An 8k gene-based SNP array was used to genotype more than 1,000 full-sibs from two intraspecific and two interspecific full-sib families of Quercus petraea and Quercus robur. A high degree of collinearity was observed between the eight parental maps of the two species. A composite map was then established with 4,261 SNP markers spanning 742 cM over the 12 linkage groups (LGs) of the oak genome. Nine genomic regions from six LGs displayed highly significant distortions of segregation. Two main hypotheses concerning the mechanisms underlying segregation distortion are discussed: genetic load vs. reproductive barriers. Our findings suggest a predominance of pre-zygotic to post-zygotic barriers.