A carrot leucine-rich-repeat protein that inhibits ice recrystallization
A carrot leucine-rich-repeat protein that inhibits ice recrystallization
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DOI：
10.1126/science.282.5386.115
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发表时间：
1998-10-02
期刊：
影响因子：
56.9
通讯作者：
Bowles, D
中科院分区：
文献类型：
--
作者：
Worrall, D;Elias, L;Bowles, D
许多适应在零度以下温度下生活的生物表达抗冻蛋白，提高其对冷冻的耐受性。尽管结构上不同，但所有抗冻蛋白都与冰表面相互作用，降低水溶液的冻结温度，并抑制冰晶生长。从胡萝卜中纯化的蛋白质与鱼的抗冻蛋白具有这些功能特征。胡萝卜互补DNA在烟草中的表达导致在温室温度下生长的植物质外体中抗冻活性的积累。胡萝卜抗冻蛋白的序列与多聚半乳糖醛酸酶抑制剂蛋白的序列相似，并且含有富含亮氨酸的重复序列。
Many organisms adapted to live at subzero temperatures express antifreeze proteins that improve their tolerance to freezing. Although structurally diverse, all antifreeze proteins interact with ice surfaces, depress the freezing temperature of aqueous solutions, and inhibit ice crystal growth. A protein purified from carrot shares these functional features with antifreeze proteins of fish. Expression of the carrot complementary DNA in tobacco resulted in the accumulation of antifreeze activity in the apoplast of plants grown at greenhouse temperatures. The sequence of carrot antifreeze protein is similar to that of polygalacturonase inhibitor proteins and contains leucine-rich repeats.