Amino acid backbone specificity of the Escherichia coli translation machinery
Amino acid backbone specificity of the Escherichia coli translation machinery
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DOI：
10.1021/ja0472174
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发表时间：
2004-10-13
影响因子：
15
通讯作者：
Cornish, VW
中科院分区：
文献类型：
--
作者：
Tan, ZP;Forster, AC;Cornish, VW
利用纯埃氏菌共译系统，我们通过测定一系列在意义密码子GUU （Val）上具有不同主链结构的酰基结合的肽合成的相对产率来测试蛋白质生物合成机制的内在特异性。结果表明，同一tRNA接头上的不同氨基酸产生的肽产量显著不同，核糖体解码aa-tRNA时氨基酸与tRNA体/反密码子之间可能产生串扰。它们进一步支持了核糖体生物合成机制的底物可塑性，并为核糖体编码聚合物合成提供了直接的候选物。
Using a pureEscherichia colitranslation system, we tested the intrinsic specificity of the protein biosynthetic machinery by determining the relative yields of peptide synthesis for incorporation of a series of acyl- 's with varied backbone structures at the sense codon GUU (Val). The results showed that different amino acids on the same tRNA adaptor give significantly different peptide yields and the potential for cross-talk between the amino acid and tRNA body/anticodon in aa-tRNA decoding by the ribosome. They further support the substrate plasticity of the ribosomal biosynthetic machinery and provide immediate candidates for ribosomally encoded polymer synthesis.