Long non-coding RNA Linc00662 promotes cell invasion and contributes to cancer stem cell-like phenotypes in lung cancer cells
Long non-coding RNA Linc00662 promotes cell invasion and contributes to cancer stem cell-like phenotypes in lung cancer cells
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DOI：
10.1093/jb/mvy078
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发表时间：
2018-12-01
影响因子：
2.7
通讯作者：
Wang, Xiumei
中科院分区：
文献类型：
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作者：
Gong, Wenjing;Su, Yi;Wang, Xiumei
长链非编码RNA（lncRNA）在调节癌细胞和癌干细胞（CSC）行为中是必不可少的。Linc00662是一种新发现的人肺癌相关lncRNA。本研究旨在探讨Linc 00662在人肺癌中的作用。通过实时定量PCR分析Linc 00662的表达水平。transwell法和划痕法检测细胞的转移和侵袭能力。肿瘤球形成实验和流式细胞术检测肿瘤干细胞的干细胞性。通过RNA免疫沉淀和RNA pulldown实验证实了Lin00662和Lin28之间的相互作用。Linc 00662过表达促进肺癌预后不良。Linc 00662可影响H1299和A549细胞的侵袭、转移和干细胞。Linc 00662与Lin28有直接的相互作用，Linc 00662的功能依赖于Lin28。我们证明Lin00662的过表达通过与人肺癌中的Lin28相互作用而增强肺癌细胞的转移和CSC干性，其可用作肺癌患者的潜在诊断和治疗靶点。
Long non-coding RNAs (lncRNAs) is essential in regulation of cancer cell and cancer stem cells (CSCs) behaviour. Linc00662 is a newly identified human lung cancer related lncRNA. In this study, we aimed to explore the function of Linc00662 in human lung cancer. The expression level of Linc00662 was analysed by quantitative real-time PCR. Cell metastasis and invasive ability were detected by transwell and scratch wound healing assays. The stemness of CSCs was shown by tumorsphere formation assay and flow cytometry. The interaction between Linc00662 and Lin28 was confirmed by RNA immunoprecipitation and RNA pulldown assay. Overexpression of Linc00662 promoted the poor prognosis of lung cancer. Cell invasion, metastasis and CSCs stemness in H1299 and A549 could be influenced by Linc00662. Linc00662 had direct interaction with Lin28, and the Linc00662 function was dependent on Lin28. We demonstrate that overexpression of Linc00662 enhances lung cancer cell metastasis and CSC stemness by interacting with Lin28 in human lung cancer, which could be utilized as a potential diagnostic and therapeutic target for lung cancer patients.