Polyribosome targeting to microtubules: enrichment of specific mRNAs in a reconstituted microtubule preparation from sea urchin embryos.
Polyribosome targeting to microtubules: enrichment of specific mRNAs in a reconstituted microtubule preparation from sea urchin embryos.
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DOI：
10.1083/jcb.127.4.973
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发表时间：
1994-11
影响因子：
7.8
通讯作者：
Suprenant, K A
中科院分区：
文献类型：
--
作者：
Hamill, D;Davis, J;Drawbridge, J;Suprenant, K A
一部分 mRNA、多聚核糖体和多聚腺苷酸结合蛋白与海胆胚胎的微管共纯化。多项证据表明，微管与核糖体的相互作用是特定的：一种独特的茎状结构似乎介导了它们的关联；通过几个纯化循环，核糖体以恒定的化学计量与微管结合；这些制剂中核糖体的存在取决于完整微管的存在。微管结合核糖体富集了五种特定的 mRNA，表明特定蛋白质的翻译可能发生在体内微管支架上。
A subset of mRNAs, polyribosomes, and poly(A)-binding proteins copurify with microtubules from sea urchin embryos. Several lines of evidence indicate that the interaction of microtubules with ribosomes is specific: a distinct stalk-like structure appears to mediate their association; ribosomes bind to microtubules with a constant stoichiometry through several purification cycles; and the presence of ribosomes in these preparations depends on the presence of intact microtubules. Five specific mRNAs are enriched with the microtubule- bound ribosomes, indicating that translation of specific proteins may occur on the microtubule scaffolding in vivo.