Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes.
Detection and Quantification of Lipid Droplets in Differentiated Human Podocytes.
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分化的人足细胞中脂滴的检测和定量。
DOI：
10.1007/978-1-4939-9488-5_17
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Al-Ali,Hassan
中科院分区：
文献类型：
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作者：
Mallela,ShamroopKumar;Patel,DevangMaheshkumar;Ducasa,GloriaMichelle;Merscher,Sandra;Fornoni,Alessia;Al-Ali,Hassan
脂滴（LD）是调节细胞内甘油三酯和其他中性脂质的储存和稳态的动态细胞器。研究表明，LD 的数量、形态和亚细胞定位在越来越多的疾病中发生了改变。因此，LD 成像和定量方法已成为检测细胞脂质代谢变化的重要工具，这可能是疾病发作或进展的重要指标。我们之前报道过代谢性和非代谢性肾病中肾小球足细胞中 LD 的积累。在这里，我们描述了一种高内涵分析 (HCA) 方法，用于自动检测和定量分化的人类足细胞中的 LD。
Lipid droplets (LDs) are dynamic organelles that regulate the storage and homeostasis of intracellular triglycerides and other neutral lipids. Studies show that the number, morphology, and subcellular localization of LDs are altered in a growing number of diseases. As such, methodologies for imaging and quantifying LDs have become essential tools for detecting changes in cellular lipid metabolism, which could be an important indicator of disease onset or progression. We previously reported on the accumulation of LDs in podocytes of the kidney glomerulus in nephrological diseases of metabolic and non-metabolic origin. Here, we describe a high-content analysis (HCA) method for automated detection and quantification of LDs in differentiated human podocytes.