Microbial characteristics in anaerobic membrane bioreactor treating domestic sewage: Effects of HRT and process performance
Microbial characteristics in anaerobic membrane bioreactor treating domestic sewage: Effects of HRT and process performance
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DOI：
10.1016/j.jes.2021.04.022
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发表时间：
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通讯作者：
Jialing Ni;Jiayuan Ji;Y. Li;K. Kubota
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作者：
Jialing Ni;Jiayuan Ji;Y. Li;K. Kubota
两个配备不同膜孔径（0.4 或 0.05 µm）的厌氧膜生物反应器 (AnMBR) 在 25°C 下运行，并注入生活废水。反应器的水力停留时间（HRT）缩短。通过 16S rRNA 基因扩增子测序对两个 AnMBR 的微生物群落进行研究，以了解 HRT 的影响。主要的古菌是一种醋酸产甲烷菌Methanosaeta。氢营养产甲烷菌的组成随 HRT 的变化而变化：HRT 较长时，甲烷杆菌的数量较多，而 HRT 较短时，未分类的甲烷菌的数量较多。厌氧菌、拟杆菌和梭状芽胞杆菌在两个反应器中均占优势，合计相对丰度超过 55%。 Anaerolineae 的相对丰度与沼气生产性能成正比。氢营养产甲烷菌或厌氧产甲烷菌种群的变化可用作过程监测的指标。当反应器稳定运行时，Anaerolineae 和 Clostridia 的相对丰度之和随着两个 AnMBR 中 HRT 的变化而略有波动。共现分析表明，在实际生活污水处理过程中，厌氧菌亚科和梭状芽胞杆菌属的操作分类单元的相对丰度在功能上是等效的。主协调分析表明，各反应器内微生物群落的变化与HRT的变化一致。此外，HRT和过程的稳定性都是维持微生物群落结构的重要因素。
Two anaerobic membrane bioreactors (AnMBRs) equipped with different membrane pore size (0.4 or 0.05 µm) were operated at 25˚C and fed with domestic wastewater. The hydraulic retention time (HRT) of the reactors was shortened. The microbial communities of the two AnMBRs were investigated by 16S rRNA gene amplicon sequencing to see the effects of HRT. The predominant Archaea was an aceticlastic methanogenMethanosaeta. The composition of hydrogenotrophic methanogens changed with the HRTs: the population ofMethanobacteriumwas higher for longer HRTs, whereas the population of unclassified Methanoregulaceae was higher for shorter HRTs. The Anaerolineae, Bacteroidia and Clostridia bacteria were dominant in both of the reactors, with a combined relative abundance of over 55%. The relative abundance of Anaerolineae was proportional to the biogas production performance. The change in the population of hydrogenotrophic methanogens or Anaerolineae can be used as an indicator for process monitoring. The sum of the relative abundance of Anaerolineae and Clostridia fluctuated slightly with changes in the HRT in both AnMBRs when the reactor was stably operated. The co-occurrence analysis revealed the relative abundance of the operational taxonomic units belonging to Anaerolineae and Clostridia was functionally equivalent during the treatment of real domestic sewage. A principal coordination analysis revealed that the changes in the microbial community in each reactor were consistent with the change of HRT. In addition, both the HRT and the stability of the process are important factors for maintaining microbial community structures.