FORMATION AND METABOLISM OF TETRAHYDRODEOXYCORTICOSTERONE BY HUMAN FECAL FLORA
FORMATION AND METABOLISM OF TETRAHYDRODEOXYCORTICOSTERONE BY HUMAN FECAL FLORA
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DOI：
10.1016/0022-4731(76)90187-4
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发表时间：
1976-01-01
影响因子：
4.1
通讯作者：
KELLY, WG
中科院分区：
文献类型：
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作者：
BOKKENHEUSER, VD;WINTER, J;KELLY, WG
根据实验条件，脱氧皮质酮(DOC)与人类粪便菌群[细菌]孵育会导致各种产物的形成。粪便菌群，稀释101-107，将DOC(16微克/毫升)还原为TH[四氢]DOC，后者进一步代谢为3α-孕烯醇酮。少量的3α-孕酮依次转化为孕二酮和3β-孕酮。另一种结构，暂定为20.X.21-二羟基-5.XI-孕烷-3酮(代谢物X)，在孵育的早期阶段通常以5-10%的产率形成。这些发现在使用纯化的THDOC和纯化的孕酮作为底物的实验中得到了证实。副滋养梭菌对A环DOC和孕酮的降解率为80%。许多无孢子厌氧菌也降低了A环中的DOC，但产率为8%。一些厌氧微生物菌株将DOC转化为代谢物X，似乎是最终产物。负责21-脱羟基的微生物还没有被分离出来。
Incubation of deoxycorticosterone (DOC) with human fecal flora [bacteria] results in the formation of a variety of products depending on the experimental conditions. Fecal flora, diluted 101-107, reduced DOC (16 .mu.g/ml) to TH[tetrahydro-]DOC which was further metabolized to 3.alpha.-pregnanolone. Small amounts of 3.alpha.-pregnanolone, in turn, were transformed to pregnandione and 3.beta.-pregnanolone. Another structure, tentatively identified as 20.xi.21-dihydroxy-5.xi.-pregnan-3 one (metabolite X) was often formed in yields of 5-10% in the early phases of incubation. These findings were corroborated in experiments using purified THDOC and purified pregnanolone as substrates. Clostridium paraputrificum reduced DOC and progesterone in ring A in yields of 80%. Many nonsporing anaerobes also reduced DOC in ring A but in yields of < 8%. Some strains of anaerobic organisms transformed DOC to metabolite X which appeared to be an endproduct. Organisms responsible for 21-dehydroxylation have not yet been isolated.