Synthesis, characterization and biocompatibility of PEGA resins.
Synthesis, characterization and biocompatibility of PEGA resins.
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DOI：
10.1002/psc.310010106
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发表时间：
1995-01-01
期刊：
影响因子：
--
通讯作者：
Bock, K
中科院分区：
文献类型：
--
作者：
Auzanneau, F I;Meldal, M;Bock, K
采用反相悬浮聚合法合成了三种高聚乙二醇含量的珠状聚乙二醇聚丙烯酰胺共聚物（PEGA），并对其进行了表征。已在树脂上合成了几种高纯度的肽。所确定的性能是氨基的负载，溶胀，珠粒尺寸分布，孔隙率，灵活性和与活性生物分子的相容性。已获得0.35 mmol/g的负载量，并且在从水到二氯甲烷的各种极性的溶剂中的溶胀是优异的。在DMSO-d 6中测定了含有肽的树脂的13 C-NMR T1弛豫时间，发现该树脂表现出与游离溶液中组分相似的行为。
Three types of beaded polyethylene glycol polyacrylamide copolymers (PEGA) with a high content of polyethylene glycol (PEG) were synthesized by inverse suspension polymerization and characterized for peptide synthesis and with respect to their physical properties. Several peptides of high purity have been synthesized on the resin. The properties which were determined were loading of amino groups, swelling, bead size distribution, porosity, flexibility and compatibility with active biomolecules. A loading of 0.35 mmol/g has been obtained and the swelling was excellent in solvents of various polarities ranging from water to dichloromethane. The 13C-NMR T1-relaxation times of a resin containing a peptide were determined in DMSO-d6 and the resin was found to exhibit a behavior similar to the components in free solution.