MUTATIONS AFFECTING REPRESSIBILITY OF ARGININE BIOSYNTHETIC ENZYMES IN SACCHAROMYCES-CEREVISIAE
MUTATIONS AFFECTING REPRESSIBILITY OF ARGININE BIOSYNTHETIC ENZYMES IN SACCHAROMYCES-CEREVISIAE
复制标题
DOI：
10.1111/j.1432-1033.1970.tb00817.x
复制
发表时间：
1970-01-01
期刊：
影响因子：
--
通讯作者：
WIAME, JM
中科院分区：
文献类型：
--
作者：
BECHET, J;GRENSON, M;WIAME, JM
< jats: p>本发明描述了一种分离酿酒酵母突变体的方法< jats: italic>，所述酿酒酵母突变体已经丧失了外源精氨酸对鸟氨酸转氨甲酰酶的抑制性。< jats: p>除了渗透性突变体，三个互补的突变类型被发现：argRI，argRII和argRIII，这是隐性的，并定义三个位点。没有证据表明这三个位点之间的任何一个连锁或与编码鸟氨酸转氨甲酰酶的基因。< jats: p>在这些基因座上携带突变的菌株无法根据表型进行区分：在基本培养基上生长后，< jats: sc>L-鸟氨甲酰基转移酶活性是野生型菌株的两倍;这些突变既不改变生长速率，也不改变对精氨酸的渗透性。< jats: p>这些突变可能会影响异源多肽脱辅基阻遏物的结构。< jats: p>二倍体中的鸟氨酸转氨甲酰酶水平与< jats: sup>调节细胞以及非调节细胞中的argF +基因的数量成比例。
< jats: p> A method is described for isolating mutants of< jats: italic> Saccharomyces cerevisiae which have lost repressibility by exogenous arginine for ornithine transcarbamylase.< jats: p> Besides permeability mutants, three complementary classes of mutations were found: argRI, argRII and argRIII which are recessive and define three loci. No evidence for a linkage between any of these three loci or with the gene coding for ornithine transcarbamylase has been obtained.< jats: p> Strains bearing mutations at either of these loci cannot be distinguished on a phenotype basis: after growth on minimal medium, the< jats: sc> l‐ornithine carbamoyl transferase activity is twice that of the wild type strain; the mutations modify neither the growth rate nor the permeability to arginine.< jats: p> The mutations might affect the structure of an hetero‐polypeptidic aporepressor.< jats: p> The level of ornithine transcarbamylase in diploids is proportional to the number of argF< jats: sup>+ genes in regulated as well as in non‐regulated cells.