Platelet-derived microparticles and soluble factors differentially regulate human endometrial epithelial cell movement
Platelet-derived microparticles and soluble factors differentially regulate human endometrial epithelial cell movement
复制标题
血小板衍生微粒和可溶性因子差异调节人子宫内膜上皮细胞运动
DOI：
10.1111/aji.12641
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发表时间：
2017
影响因子：
3.6
通讯作者：
Fujiwara H
中科院分区：
文献类型：
--
作者：
Suginami K;Sato Y;Horie A;Matsumoto H;Tani H;Mizumoto Y;Ono M;Matsuoka A;Kyo S;Araki Y;Konishi I;Fujiwara H
问题我们以前提出血小板在月经期间促进上皮再形成。由于细胞运动是组织重塑过程中重要的细胞行为之一，我们研究了血小板对子宫内膜上皮细胞侵袭的影响。使用人子宫内膜上皮细胞来源的永生化细胞系，EM-E6/E7/hTERT细胞，我们检查了血小板和血小板来源的条件培养基（有或没有微粒）对EM-E6/E7/hTERT细胞的形态学和侵袭特性的影响。而去除微粒的条件培养基促进了它们的侵袭，而没有任何皮质环形成的显著变化。结论这些结果支持了我们以前的提议，并新提出了血小板的双重作用：血小板衍生的可溶性因子，可促进远端区域的细胞运动，以及通过近端区域的子宫内膜上皮细胞诱导再上皮化的微粒。
ProblemWe previously proposed that platelets promote re‐epithelialization during menstruation. As cell movement is one of the important cell behaviors in the process of tissue remodeling, we examined the effects of platelets on endometrial epithelial cell invasion.Method of StudyThe platelets were isolated from healthy women. Using a human endometrial epithelial cell‐derived immortalized cell line, EM‐E6/E7/hTERT cells, we examined the effects of platelets and platelet‐derived condition media with or without microparticles on the morphological and invasive properties of EM‐E6/E7/hTERT cells.ResultsPlatelets and microparticle‐containing conditioned media inhibited Matrigel invasion by EM‐E6/E7/hTERT cells along with an increase in cortical ring formation, whereas microparticle‐depleted conditioned media promoted their invasion without any significant changes of cortical ring formation.ConclusionThese results support our previous proposal and newly suggest the dual roles of platelets: platelet‐derived soluble factors that promote cell movement in the distant area, and microparticles that induce re‐epithelialization by endometrial epithelial cells in the proximal area.