RESTRICTIONS TO FLOOR PLATE INDUCTION BY HEDGEHOG AND WINGED-HELIX GENES IN THE NEURAL-TUBE OF FROG EMBRYOS
RESTRICTIONS TO FLOOR PLATE INDUCTION BY HEDGEHOG AND WINGED-HELIX GENES IN THE NEURAL-TUBE OF FROG EMBRYOS
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DOI：
10.1006/mcne.1995.1011
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发表时间：
1995-04-01
影响因子：
3.5
通讯作者：
ROELINK, H
中科院分区：
文献类型：
--
作者：
ALTABA, ARI;JESSEL, TM;ROELINK, H
脊椎动物刺猬家族的细胞间信号分子和翼螺旋家族的转录因子与底板发育有关。我们研究了在青蛙胚胎的神经板和神经管中错误表达脊椎动物hedgehog基因vhh-1 （sonic hedgehog, shh）和翼螺旋基因HNF-3 β的后果。这两种基因中的任何一种的错误表达都会在异位位置诱导底板分化。然而，异位底板诱导对vhh-1和HNF-3 β的响应在时间和空间上受到限制。神经板分期未见底板异位分化。神经管闭合后，发现异位底板分化，但主要局限于神经管背侧区域。因此，翼螺旋基因和脊椎动物刺猬基因在体内诱导底板分化的能力可能受到底板分化时间和位置的附加信号的限制。(C) 1995学术出版社，Inc。
Intercellular signaling molecules of the vertebrate hedgehog family and transcription factors of the winged-helix family have been implicated in floor plate development. We have examined the consequences of misexpressing the vertebrate hedgehog gene vhh-1 (sonic hedgehog, shh) and the winged-helix gene HNF-3 beta in the neural plate and neural tube of frog embryos. Misexpression of either of these genes induces floor plate differentiation at ectopic locations. However, ectopic floor plate induction in response to both vhh-1 and HNF-3 beta was temporally and spatially restricted. At neural plate stages, ectopic floor plate differentiation was not detected. After neural tube closure, ectopic floor plate differentiation was detected but was restricted predominantly to the dorsal region of the neural tube. The ability of winged-helix and vertebrate hedgehog genes to induce floor plate differentiation in vivo may, therefore, be constrained by additional signals that specify the time and position of floor plate differentiation. (C) 1995 Academic Press, Inc.