Early human preantral follicles have relaxin and relaxin receptor (LGR7), and relaxin promotes their development
Early human preantral follicles have relaxin and relaxin receptor (LGR7), and relaxin promotes their development
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DOI：
10.1210/jc.2004-0130
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发表时间：
2005-01-01
影响因子：
5.8
通讯作者：
Kawarabayashi, T
中科院分区：
文献类型：
--
作者：
Shirota, K;Tateishi, K;Kawarabayashi, T
早期卵泡发育的调控机制尚不清楚。尽管松弛素是一种在许多组织中控制细胞增殖和分化的肽，但其在人类卵泡发育中的作用尚不清楚。在这项研究中，我们培养切片的人卵巢皮质组织在存在和不存在重组人松弛素。在妇科剖腹手术或腹腔镜检查中通过活检获得卵巢组织（14名女性;平均年龄+/- SEM，29.0 +/- 6.1岁;范围，17-37岁）。与对照组相比，松弛素组次级卵泡比例显著增加（14.5% vs.5.0%，P < 0.01），原始卵泡比例显著降低（30.1% vs.47.4%，P < 0.05）。免疫细胞化学研究显示，松弛素原和抗松弛素抗体的抗C-肽的松弛素在卵母细胞和平坦的前颗粒细胞和颗粒细胞的原始，初级和次级卵泡的本地化。松弛素受体LGR 7的存在下，观察到在平坦的前颗粒和颗粒细胞的原始，初级和次级卵泡的免疫细胞化学和原位杂交分析。这些结果表明，松弛素通过其受体在卵泡发育的早期阶段发挥作用。
The regulatory mechanisms of early follicle development are not clearly understood. Although relaxin is a peptide that controls cell proliferation and differentiation in many tissues, its role in human follicular development is unclear. In this study we cultured slices of human ovarian cortical tissue in the presence and absence of recombinant human relaxin. Ovarian tissue was obtained by biopsy during gynecological laparotomy or laparoscopy (14 women; mean age +/- SEM, 29.0 +/- 6.1 yr; range, 17-37 yr). A significantly higher proportion of secondary follicles (14.5% vs. 5.0% in the control group; P < 0.01) and a significantly decreased proportion of primordial follicles (30.1% vs. 47.4% in the control group; P < 0.05) were found in tissues cultured with relaxin for 7 d. Immunocytochemical studies with the anti-C-peptide of prorelaxin and antirelaxin antibodies revealed the localization of relaxin in the oocyte and in flat pregranulosa and granulosa cells of primordial, primary, and secondary follicles. The presence of the relaxin receptor LGR7 was observed in flat pregranulosa and granulosa cells of primordial, primary, and secondary follicles by immunocytochemical and in situ hybridization analyses. These results suggest that relaxin plays a role through its receptor during the early stage of follicle development.