SERCA2a gene transfer improves electrocardiographic performance in aged mdx mice.
SERCA2a gene transfer improves electrocardiographic performance in aged mdx mice.
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DOI：
10.1186/1479-5876-9-132
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发表时间：
2011-08-11
影响因子：
7.4
通讯作者：
Duan D
中科院分区：
文献类型：
--
作者：
Shin JH;Bostick B;Yue Y;Hajjar R;Duan D
Cardiomyocyte calcium overloading has been implicated in the pathogenesis of Duchenne muscular dystrophy (DMD) heart disease. The cardiac isoform of sarcoplasmic reticulum calcium ATPase (SERCA2a) plays a major role in removing cytosolic calcium during heart muscle relaxation. Here, we tested the hypothesis that SERCA2a over-expression may mitigate electrocardiography (ECG) abnormalities in old female mdx mice, a murine model of DMD cardiomyopathy. 1 × 1012 viral genome particles/mouse of adeno-associated virus serotype-9 (AAV-9) SERCA2a vector was delivered to 12-m-old female mdx mice (N = 5) via a single bolus tail vein injection. AAV transduction and the ECG profile were examined eight months later. The vector genome was detected in the hearts of all AAV-injected mdx mice. Immunofluorescence staining and western blot confirmed SERCA2a over-expression in the mdx heart. Untreated mdx mice showed characteristic tachycardia, PR interval reduction and QT interval prolongation. AAV-9 SERCA2a treatment corrected these ECG abnormalities. Our results suggest that AAV SERCA2a therapy may hold great promise in treating dystrophin-deficient heart disease.
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DOI：
10.1073/pnas.0908540107
发表时间：
2010-01-26
影响因子：
11.1
作者：
Fauconnier, Jeremy;Thireau, Jerome;Lacampagne, Alain
通讯作者：
Lacampagne, Alain
DOI：
10.1007/978-1-61737-982-6_6
发表时间：
2011-01-01
期刊：
MUSCLE GENE THERAPY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Bostick, Brian;Yue, Yongping;Duan, Dongsheng
通讯作者：
Duan, Dongsheng
DOI：
10.1111/j.1365-201x.2005.01450.x
发表时间：
2005-07-01
期刊：
ACTA PHYSIOLOGICA SCANDINAVICA
影响因子：
--
作者：
Divet, A;Lompré, AM;Huchet-Cadiou, C
通讯作者：
Huchet-Cadiou, C
影响因子：
2.7
作者：
Constantin, Bruno;Sebille, Stephane;Cognard, Christian
通讯作者：
Cognard, Christian
影响因子：
4
作者：
Fanchaouy M;Polakova E;Jung C;Ogrodnik J;Shirokova N;Niggli E
通讯作者：
Niggli E