Proteomic quantitative study of dorsal root ganglia and sciatic nerve in type 2 diabetic mice.
Proteomic quantitative study of dorsal root ganglia and sciatic nerve in type 2 diabetic mice.
复制标题
DOI：
10.1016/j.molmet.2021.101408
复制
发表时间：
2022-01
影响因子：
8.1
通讯作者：
Bosch A
中科院分区：
文献类型：
--
作者：
Leal-Julià M;Vilches JJ;Onieva A;Verdés S;Sánchez Á;Chillón M;Navarro X;Bosch A
Peripheral neuropathy is the most common and debilitating complication of type 2 diabetes, leading to sensory loss, dysautonomia, hyperalgesia, and spontaneous noxious sensations. Despite the clinical and economic burden of diabetic neuropathy, no effective treatment is available. More preclinical research must be conducted in order to gain further understanding of the aetiology of the disease and elucidate new therapeutic targets. The proteome of lumbar dorsal root ganglia and sciatic nerve of BKS-db/db mice, which contain a mutation of the leptin receptor and are an established type 2 diabetes model, was characterized for the first time by tandem mass tag labelling and mass spectrometry analysis. Proteomic analysis showed differentially expressed proteins grouped into functional clusters in db/db peripheral nerves compared to control mice, underlining reduced glycolytic and TCA cycle metabolism, higher lipid catabolism, upregulation of muscle-like proteins in DRG and downregulation in SCN, increased cytoskeleton-related proteins, a mild dysregulation of folding chaperones, activation of acute-phase and inflammatory response, and alterations in glutathione metabolism and oxidative stress related proteins. Our data validate previous transcriptomic and metabolomic results and uncover new pathways altered in diabetic neuropathy. Our results point out that energetic deficiency could represent the main mechanism of neurodegeneration observed in diabetic neuropathy. These findings may provide important information to select appropriate targets to develop new therapeutic strategies. Unprecedent proteomic analysis of DRG and sciatic nerve from db/db mouse. Oxidative stress, inflammation and cytoskeleton abnormalities are linked to neuropathy. Low ATP and energetic imbalance mediate neurodegeneration in diabetic neuropathy.
登录
查看更多内容
DOI：
10.1530/joe-12-0356
发表时间：
2013-01
期刊：
The Journal of endocrinology
影响因子：
--
作者：
Hinder LM;Vivekanandan-Giri A;McLean LL;Pennathur S;Feldman EL
通讯作者：
Feldman EL
影响因子：
81.5
作者：
Feldman, Eva L.;Callaghan, Brian C.;Viswanathan, Vijay
通讯作者：
Viswanathan, Vijay
影响因子：
64.8
作者：
Baur, Joseph A.;Pearson, Kevin J.;Sinclair, David A.
通讯作者：
Sinclair, David A.
影响因子：
3.8
作者：
Homs, Judit;Ariza, Lorena;Navarro, Xavier
通讯作者：
Navarro, Xavier
影响因子：
4.8
作者：
Jo, SH;Son, MK;Huhe, TL
通讯作者：
Huhe, TL