The transcription factor OsNAC4 is a key positive regulator of plant hypersensitive cell death
The transcription factor OsNAC4 is a key positive regulator of plant hypersensitive cell death
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DOI：
10.1038/emboj.2009.39
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发表时间：
2009-04-08
期刊：
影响因子：
11.4
通讯作者：
Che, Fang-Sik
中科院分区：
文献类型：
--
作者：
Kaneda, Takashi;Taga, Yuri;Che, Fang-Sik
超敏反应（HR）是植物免疫反应和一种程序性细胞死亡的共同特征。但是，关于HR细胞死亡的诱导机制知之甚少。我们报告说，编码植物特异性转录因子的OSNAC4的过表达导致HR细胞死亡伴随着质膜完整性，核DNA碎片和典型的形态变化的丧失。在OSNAC4敲低线中，HR细胞死亡对无毒细菌菌株的响应显着降低。通过无毒病原体识别信号诱导后，以磷酸化依赖性方式将OSNAC4转移到核中。微阵列分析表明，编码Ca2+依赖性核酸酶在内的139个基因的表达在HR细胞死亡期间的OSNAC4敲低线和控制线之间有所不同。在诱导HR细胞死亡期间，OSHSP90参与了质膜完整性的丧失，而IREN会导致核DNA碎片。总体而言，我们的结果表明，在HR细胞死亡期间发生的两个重要事件由独立途径调节。
The hypersensitive response (HR) is a common feature of plant immune responses and a type of programmed cell death. However, little is known about the induction mechanism of HR cell death. We report that overexpression of OsNAC4, which encodes a plant-specific transcription factor, leads to HR cell death accompanied by the loss of plasma membrane integrity, nuclear DNA fragmentation and typical morphological changes. In OsNAC4 knock-down lines, HR cell death is markedly decreased in response to avirulent bacterial strains. After induction by an avirulent pathogen recognition signal, OsNAC4 is translocated into the nucleus in a phosphorylation- dependent manner. A microarray analysis showed that the expression of 139 genes including OsHSP90 and IREN, encoding a Ca2+-dependent nuclease, were different between the OsNAC4 knock-down line and control line during HR cell death. During the induction of HR cell death, OsHSP90 is involved in the loss of plasma membrane integrity, whereas IREN causes nuclear DNA fragmentation. Overall, our results indicate that two important events occurring during HR cell death are regulated by independent pathways.