Mapping of QTLs associated with important agronomic traits using three populations derived from a super hybrid rice Xieyou9308
Mapping of QTLs associated with important agronomic traits using three populations derived from a super hybrid rice Xieyou9308
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利用超级杂交稻协优9308的三个群体绘制与重要农艺性状相关的QTL
DOI：
10.1007/s10681-011-0456-4
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发表时间：
2012-03-01
期刊：
影响因子：
1.9
通讯作者：
Cheng, Shihua
中科院分区：
文献类型：
--
作者：
Liang, Yongshu;Zhan, Xiaodeng;Cheng, Shihua
协优9308是我国1996年育成的第一个商品化超级杂交稻。为阐明小麦高产的遗传机制，利用小麦保持系协青早B（XQZB）与恢复系中恢9308（ZH9308）杂交构建重组自交系（RIL）群体，并利用回交F1（BCF 1）群体，对10个重要农艺性状进行QTL定位（分蘖数（TN）、抽穗期（HD）和单株籽粒产量（GYPP）等）。通过广义遗传力（hB2）和表型相关系数（PCC）分析，两个群体的BCF 1表现与其亲本RIL表现密切相关，但QTL分析未证实这一点。利用WinQTLCart2.50软件对RIL和2个BCF1群体的10个性状分别检测到21个加性效应主效QTL（M-QTL）、22个显性效应M-QTL和19个显性效应M-QTL。其中，3个QTL在RIL和1个BCF 1群体中重复检测到21个（qHD7a、qPPP3a和qPL10）QTL，在2个BCF 1群体中重复检测到10个控制4个性状的QTL，在2个BCF 1群体中重复检测到9个控制2个以上性状的QTL，加性效应QTL和显性效应QTL在3个群体中均未检测到加性-加性效应和显性-显性效应的上位性QTL。本研究为进一步精细定位和定位克隆重要农艺性状相关的M-QTL奠定了基础。
Xieyou9308 is the first commercial super hybrid rice released in 1996 in China. To clarify its genetic mechanism underlying high yield potential, a recombinant inbred line (RIL) population derived from the cross between the maintainer line XieqingzaoB (XQZB) and the restorer line Zhonghui9308 (ZH9308) and two derived backcross F1(BCF1) populations were developed for the identification of quantitative trait loci (QTLs) related to ten important agronomic traits (tiller number (TN), heading date (HD), and grain yield per plant (GYPP), etc.). The BCF1performance was closely correlated with the performance of their parental RILs according to both the analysis of broad-sense heritability (hB2) and phenotypic correlation coefficient (PCC) in the two BCF1populations, but not proved by QTL analysis. A total of 21 additive-effect main QTLs (M-QTLs), 22 dominant-effect M-QTLs, and 19 dominant-effect M-QTLs were detected with the WinQTLCart 2.50 software for the ten traits in the RIL and two BCF1populations, respectively. Of theses, three QTLs (qHD7a,qPPP3a, andqPL10) of 21 were detected repeatedly in the RIL and one BCF1populations, ten QTLs underlying four traits were only detected repeatedly in two BCF1populations, and nine QTLs controlling more than two traits were detected repeatedly, the additive-effect QTLs and dominant-effect QTLs play an important role in the performance of agronomic traits and no epistatic QTL of additive by additive effect and dominant by dominant-effect was detected for all traits in three populations. This research is valuable for M-QTL related to important agronomic trait in future fine mapping and positional cloning.