Nitidine chloride inhibits renal cancer cell metastasis via suppressing AKT signaling pathway
Nitidine chloride inhibits renal cancer cell metastasis via suppressing AKT signaling pathway
复制标题
氯化两面针碱通过抑制 AKT 信号通路抑制肾癌细胞转移
DOI：
10.1016/j.fct.2013.07.062
复制
发表时间：
2013-10-01
影响因子：
4.3
通讯作者：
Liu, Zhaoxu
中科院分区：
文献类型：
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作者：
Fang, Zhiqing;Tang, Yueqing;Liu, Zhaoxu
氯化两面针碱（NC）已被证明对各种肿瘤具有抗癌作用。然而，NC是否能发挥抗肾癌细胞转移的作用及其机制尚不清楚。在这项工作中，我们的数据表明，NC对肾癌细胞在体外的抗转移作用。划痕实验和transwell实验发现，NC能有效抑制786-O和A498细胞的迁移和侵袭。从机制上讲，我们提出，NC显着降低AKT的磷酸化，伴随着MMP-2和MMP-9的下调。AKT特异性抑制剂LY 294002可增强NC的抗肿瘤转移作用，提示NC抑制肾癌细胞转移可能部分通过抑制Ala活性。综上所述，我们的研究结果表明，NC可以开发为一种潜在的抗肾癌转移剂。(C)2013爱思唯尔有限公司版权所有。
Nitidine Chloride (NC) has been shown to have anti-cancer effects on various tumors. However, whether NC could exert anti-metastasis activity in renal cancer cells and the underlying mechanisms have not been elucidated. In this work, our data demonstrated the anti-metastasis effects of NC on renal cancer cells in vitro. With scratch assay and transwell assays, we found that NC potently suppressed the migration and invasion of 786-O and A498 cells. Mechanistically, we presented that NC significantly decreased phosphorylation of AKT, accompanied by down-regulation of MMP-2 and MMP-9. Furthermore, a specific AKT inhibitor, LY294002, could enhance the anti-metastasis effects of NC, which indicated that NC suppressed metastasis of renal cancer cells partly via inhibition of Ala activity. Taken together, our results imply that NC can be developed as a potential anti-metastasis agent to renal cancer. (C) 2013 Elsevier Ltd. All rights reserved.