How Escherichia coli lands and forms cell clusters on a surface: a new role of surface topography.
How Escherichia coli lands and forms cell clusters on a surface: a new role of surface topography.
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DOI：
10.1038/srep29516
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发表时间：
2016-07-14
影响因子：
4.6
通讯作者：
Ren D
中科院分区：
文献类型：
--
作者：
Gu H;Chen A;Song X;Brasch ME;Henderson JH;Ren D
使用 5 μm 高的聚二甲基硅氧烷 (PDMS) 线图案研究了生物膜形成过程中细菌对表面形貌的反应。研究发现，当图案变窄时，附着在突出线图案顶部的大肠杆菌细胞会更垂直于线图案的方向排列。一致地，与平面 PDMS 相比，5μm 宽线图案上单位面积的细胞簇形成减少了 14 倍。与减少的集落形成形成对比的是，附着在狭窄图案上的细胞更长并且具有更高的转录活性，这表明这种不利的地形可能会给附着细胞带来压力。突变体研究的结果表明，鞭毛运动与观察到的细胞对狭窄图案的方向偏好有关，这通过细胞在不同表面形貌上定居之前旋转模式的变化得到了证实。这些发现催生了一套用于创建防污拓扑的新设计原则，并使用 10μm 高的六边形图案进行了验证。
Bacterial response to surface topography during biofilm formation was studied using 5 μm tall line patterns of poly (dimethylsiloxane) (PDMS). Escherichia coli cells attached on top of protruding line patterns were found to align more perpendicularly to the orientation of line patterns when the pattern narrowed. Consistently, cell cluster formation per unit area on 5 μm wide line patterns was reduced by 14-fold compared to flat PDMS. Contrasting the reduced colony formation, cells attached on narrow patterns were longer and had higher transcriptional activities, suggesting that such unfavorable topography may present a stress to attached cells. Results of mutant studies indicate that flagellar motility is involved in the observed preference in cell orientation on narrow patterns, which was corroborated by the changes in cell rotation pattern before settling on different surface topographies. These findings led to a set of new design principles for creating antifouling topographies, which was validated using 10 μm tall hexagonal patterns.