Biological length scale topography enhances cell-substratum adhesion of human corneal epithelial cells
Biological length scale topography enhances cell-substratum adhesion of human corneal epithelial cells
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DOI：
10.1242/jcs.01146
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发表时间：
2004-07-01
影响因子：
4
通讯作者：
Murphy, CJ
中科院分区：
文献类型：
--
作者：
Karuri, NW;Liliensiek, S;Murphy, CJ
基底膜具有丰富的三维纳米级地形，提供物理刺激，可能调节细胞-基质粘附。我们已经研究了细胞-基质粘附强度在纳米尺度上的地形特征与天然基膜相似。SV40人角膜上皮细胞受到定义明确的流体剪切冲击，并监测细胞脱离。我们使用x射线光刻技术创造了具有均匀凹槽和脊的硅基，其间距尺寸为400-4000 nm。对孵育24小时的细胞进行F-actin标记，结果显示，无论间距大小，在有图案的表面上排列和伸长的细胞的百分比是相同的。相反，在最大的流体剪切下，观察到细胞粘附的双相趋势，细胞最粘附于较小的特征。在粘附实验结束时，400 nm间距的粘附细胞百分比最高。在评估的最大特征（4000 nm间距）中，基质地形的影响消失了。对细胞流动过程和流动后的定性和定量分析表明，在400 nm间距上排列和拉长的细胞比在更大的图案上排列的细胞粘附更紧密。选择原代培养的人角膜上皮细胞进行实验，结果与SV40人角膜上皮细胞相似。这些发现与组织工程和假肢设计中细胞-生物材料相互作用的解释有关。
The basement membrane possesses a rich 3-dimensional nanoscale topography that provides a physical stimulus, which may modulate cell-substratum adhesion. We have investigated the strength of cell-substratum adhesion on nanoscale topographic features of a similar scale to that of the native basement membrane. SV40 human corneal epithelial cells were challenged by well-defined fluid shear, and cell detachment was monitored. We created silicon substrata with uniform grooves and ridges having pitch dimensions of 400-4000 nm using X-ray lithography. F-actin labeling of cells that had been incubated for 24 hours revealed that the percentage of aligned and elongated cells on the patterned surfaces was the same regardless of pitch dimension. In contrast, at the highest fluid shear, a biphasic trend in cell adhesion was observed with cells being most adherent to the smaller features. The 400 nm pitch had the highest percentage of adherent cells at the end of the adhesion assay. The effect of substratum topography was lost for the largest features evaluated, the 4000 nm pitch. Qualitative and quantitative analyses of the cells during and after flow indicated that the aligned and elongated cells on the 400 nm pitch were more tightly adhered compared to aligned cells on the larger patterns. Selected experiments with primary cultured human corneal epithelial cells produced similar results to the SV40 human corneal epithelial cells. These findings have relevance to interpretation of cell-biomaterial interactions in tissue engineering and prosthetic design.