Bovine oviductal epithelial cells (BOEC) and oviducts: I. For embryo culture. II. Using SEM for studying interactions with spermatozoa.
Bovine oviductal epithelial cells (BOEC) and oviducts: I. For embryo culture. II. Using SEM for studying interactions with spermatozoa.
复制标题
牛输卵管上皮细胞 (BOEC) 和输卵管：I. 用于胚胎培养。
DOI：
10.1002/jemt.1070310608
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Foote,RH
中科院分区：
文献类型：
--
作者：
Suzuki,H;Foote,RH
输卵管在生殖中起着重要的作用。它是一个动态器官，选择性地允许少数精子获得能力，并到达排卵后继续成熟的卵母细胞。然后，在受精后，胚胎在到达子宫之前经历卵裂。从卵母细胞的体外研究以及精子与输卵管细胞的相互作用中已经获得了广泛的信息。牛输卵管上皮细胞(BOEC)单层以简单的培养液提供了一个环境，在这个环境中，受精卵可以在6天内培养到囊胚，细胞数量基本上相当于供体动物完全生长的囊胚。这些药物在移植时会产生正常的妊娠率。目前正在测试的简单无蛋白培养液有望阐明植入前胚胎的特殊要求，这些条件为体外受精和胚胎基因工程的许多相关研究提供了便利。本文的第二部分是从扫描电子显微镜的角度对新鲜和冷冻解冻的公牛精子与BOEC和完整输卵管的相互作用进行了广泛的研究。这两种类型的输卵管细胞都在39℃下分别孵育0、3、6和9小时，材料来自排卵期奶牛。精子立即附着在两种类型的上皮上，并在3小时内达到高峰。它们主要是在完整的输卵管的沟槽中发现的。精子头部前部迅速出现分泌液滴，3小时内顶体出现明显变化，与体内报道的相似。冷冻解冻的精子变化更快。©1995 Wiley-Liss公司
The oviduct (uterine tube) plays a major role in reproduction. It is a dynamic organ which selectively permits a few sperm to undergo capacitation and reach the oocyte which has continued to undergo maturation following ovulation. Then following fertilization the embryo undergoes cleavage before arriving in the uterus. Extensive information has become available from in vitro studies on oocytes as well as spermatozoal interactions with oviductal cells. Bovine oviduct epithelial cell (BOEC) monolayers with simple media provide an environment in which zygotes can be cultured to blastocysts in 6 days with cell numbers essentially equivalent to blastocysts grown totally in the donor animal. These yield normal pregnancy rates upon transfer. The simple protein‐free media currently under test hold promise for elucidating specific requirements of the preimplantation embryo and these defined conditions facilitate many related studies on in vitro fertilization and genetic engineering of embryos.The second part of this paper is an extensive study on the interaction of fresh and frozen‐thawed bull spermatozoa with BOEC and segments of intact oviducts as viewed by SEM. Both types of oviductal cells were incubated at 39°C for 0, 3, 6, and 9 hours, using material obtained from periovulatory cows. Sperm attached immediately to both types of epithelium and reached a peak at 3 hours. They were found primarily in the furrows of the intact oviducts. Secretory droplets appeared rapidly on the anterior portion of the sperm head and acrosomal changes were evident in 3 hours, similar to those reported in vivo. Changes were more rapid with frozen‐thawed sperm. © 1995 Wiley‐Liss, Inc.