Intracellular symbionts and other bacteria associated with deer ticks (Ixodes scapularis) from Nantucket and Wellfleet, Cape Cod, Massachusetts
Intracellular symbionts and other bacteria associated with deer ticks (Ixodes scapularis) from Nantucket and Wellfleet, Cape Cod, Massachusetts
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DOI：
10.1128/aem.70.1.616-620.2004
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发表时间：
2004-01-01
影响因子：
4.4
通讯作者：
Benson, DR
中科院分区：
文献类型：
--
作者：
Benson, MJ;Gawronski, JD;Benson, DR
对采集自马萨诸塞州楠塔基特岛和科德角韦利特的7只鹿蜱（Ixodes scapularis）的16S rRNA基因进行PCR扩增、克隆和测序，评估了与鹿蜱相关的细菌多样性。获得的大部分序列来自革兰氏阴性变形菌。检测到4种细胞内细菌，包括埃利希体、立克次体和沃尔巴克氏体菌株，以及一种与细胞吞噬体-黄杆菌-拟杆菌群有关的细胞内昆虫共生体。在除一只蜱外的所有蜱身上也检测到鞘单菌科的几个菌株。研究结果对野外与肩胛骨蜱相关的细菌多样性有一定的了解。
The diversity of bacteria associated with the deer tick (Ixodes scapularis) was assessed using PCR amplification, cloning, and sequencing of 16S rRNA genes originating from seven ticks collected from Nantucket Island and Wellfleet, Cape Cod, Mass. The majority of sequences obtained originated from gram-negative proteobacteria. Four intracellular bacteria were detected including strains of Ehrlichia, Rickettsia, and Wolbachia and an organism related to intracellular insect symbionts from the Cytophaga-Flavobacterium-Bacteroides group. Several strains of members of the Sphingomonadaceae were also detected in all but one tick. The results provide a view of the diversity of bacteria associated with I. scapularis ticks in the field.