Isolation and characterization of a relevant Aspergillus fumigatus antigen with IgG- and IgE-binding activity.
Isolation and characterization of a relevant Aspergillus fumigatus antigen with IgG- and IgE-binding activity.
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DOI：
10.1159/000234568
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
V. Kurup;M. Ramasamy;P. Greenberger;J. Fink
中科院分区：
文献类型：
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作者：
V. Kurup;M. Ramasamy;P. Greenberger;J. Fink
从烟曲霉菌丝体的细胞质提取物中分离出免疫相关的抗原级分。通过伴刀豆球蛋白-A亲和层析和疏水相互作用层析获得级分。在聚丙烯酰胺凝胶电泳中检测到两条蛋白带，而在交叉免疫电泳中检测到两个沉淀素峰。当测试对患者的血清中的蛔虫引起的疾病，馏分显示IgG和IgE抗体结合活性。过敏性支气管肺曲霉病（ABPA）患者的所有血清研究显示高IgG和IgE抗体滴度，而曲霉瘤和囊性纤维化与ABPA患者的血清表现出高IgG滴度和IgE滴度只有中度增加。其他组的血清中均未显示针对该抗原的任何显著抗体滴度。由于其结合IgG和IgE抗体，该馏分被发现是有用的，在免疫诊断的蛔虫引起的疾病。
An immunologically relevant antigen fraction was isolated from a cytoplasmic extract of Aspergillus fumigatus mycelium. The fraction was obtained by concanavalin-A affinity chromatography and hydrophobic interaction chromatography. Two protein bands were discernible in polyacrylamide gel electrophoresis while two precipitin peaks were detected in crossed immunoelectrophoresis. When tested against sera from patients with Aspergillus-induced diseases, the fraction showed both IgG and IgE antibody-binding activity. All of the sera studied from patients with allergic bronchopulmonary aspergillosis (ABPA) showed high IgG and IgE antibody titers, while sera from patients with aspergilloma and cystic fibrosis with ABPA demonstrated high IgG titers and only a moderate increase in IgE titers. None of the other groups showed any significant antibody titers against this antigen in their sera. Because of its binding to both IgG and IgE antibodies this fraction was found to be useful in the immunodiagnosis of Aspergillus-induced diseases.