A significant number of human immunodeficiency virus epitope-specific cytotoxic T lymphocytes detected by tetramer binding do not produce gamma interferon
A significant number of human immunodeficiency virus epitope-specific cytotoxic T lymphocytes detected by tetramer binding do not produce gamma interferon
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DOI：
10.1128/jvi.74.21.10249-10255.2000
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发表时间：
2000-11-01
影响因子：
5.4
通讯作者：
Mulligan, MJ
中科院分区：
文献类型：
--
作者：
Goepfert, PA;Bansal, A;Mulligan, MJ
尽管细胞毒性T淋巴细胞（CTL）反应在人类免疫缺陷病毒（HIV）疾病发病机制中似乎起着重要作用，但其测量依赖于各种不同的技术。我们利用三种不同的方法来检测一组HIV感染者中的CTL，这些HIV感染者也是人类白细胞抗原A2（HLA-A2）阳性。在不同的CTL检测中，只有当Gag表位特异性HLA A*0201限制性四聚体检测与用Gag表位刺激后进行的ELISPOT检测进行比较时才发现相关性;然而，这种相关性具有边缘统计学意义。平均而言，四聚体试剂检测到的细胞数量是ELISPOT检测产生γ干扰素的细胞数量的10倍。这种CTL检测比较的影响和HIV特异性CD 8（+）T淋巴细胞表型差异的可能性进行了讨论。
Despite the seemingly important role of cytotoxic T-lymphocyte (CTL) responses in human immunodeficiency virus (HIV) disease pathogenesis, their measurement has relied on a variety of different techniques. We utilized three separate methodologies for the detection of CTLs in a cohort of HIV-infected individuals who were also human leukocyte antigen A2 (HLA-A2) positive. Among the different CTL assays, a correlation was seen only when the Gag epitope-specific HLA A*0201-restricted tetramer assay was compared with the ELISPOT assay performed after stimulation with the Gag epitope; however, this correlation was of borderline statistical significance. On average, the tetramer reagent detected a 10-fold-higher number of cells than were seen to produce gamma interferon by the ELISPOT assay. The implications of this CTL assay comparison and the possibility of phenotypic differences in HIV-specific CD8(+) T lymphocytes are discussed.