Nonvesicular sterol movement from plasma membrane to ER requires oxysterol-binding protein-related proteins and phosphoinositides.
Nonvesicular sterol movement from plasma membrane to ER requires oxysterol-binding protein-related proteins and phosphoinositides.
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DOI：
10.1083/jcb.200510084
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发表时间：
2006-04-10
期刊：
影响因子：
--
通讯作者：
Prinz WA
中科院分区：
文献类型：
--
作者：
Raychaudhuri S;Im YJ;Hurley JH;Prinz WA
类固醇通过非囊泡途径在细胞膜之间移动，其功能知之甚少。在酵母中，一种这样的途径将固醇从质膜（PM）转移到内质网（ER）。我们表明，这种运输需要氧固醇结合蛋白（OSBP）相关蛋白（ORP），这是一个大家族的保守的脂质结合蛋白。我们证明，这个家庭的代表性成员，Osh 4p/Kes 1 p，特别有利于胆固醇和麦角固醇在体外膜之间的非囊泡转移。此外，Osh 4p在含有磷酸肌醇（PIP）的膜之间更迅速地转移固醇，表明PIP通过ORP调节固醇转运。我们证实了这一点，表明PM到ER固醇运输显着放缓突变体与PIP生物合成的条件缺陷。我们的研究结果认为，氧化还原酶之间的细胞室和甾醇的运输和细胞内分布的PIP调节甾醇。
Sterols are moved between cellular membranes by nonvesicular pathways whose functions are poorly understood. In yeast, one such pathway transfers sterols from the plasma membrane (PM) to the endoplasmic reticulum (ER). We show that this transport requires oxysterol-binding protein (OSBP)–related proteins (ORPs), which are a large family of conserved lipid-binding proteins. We demonstrate that a representative member of this family, Osh4p/Kes1p, specifically facilitates the nonvesicular transfer of cholesterol and ergosterol between membranes in vitro. In addition, Osh4p transfers sterols more rapidly between membranes containing phosphoinositides (PIPs), suggesting that PIPs regulate sterol transport by ORPs. We confirmed this by showing that PM to ER sterol transport slows dramatically in mutants with conditional defects in PIP biosynthesis. Our findings argue that ORPs move sterols among cellular compartments and that sterol transport and intracellular distribution are regulated by PIPs.
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影响因子：
7.8
作者：
Li, Xinmin;Rivas, Marcos P;Fang, Min;Marchena, Jennifer;Mehrotra, Bharat;Chaudhary, Anu;Feng, Li;Prestwich, Glenn D;Bankaitis, Vytas A
通讯作者：
Bankaitis, Vytas A
DOI：
10.1083/jcb.109.1.61
发表时间：
1989-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Doms RW;Russ G;Yewdell JW
通讯作者：
Yewdell JW
影响因子：
11.4
作者：
Audhya, A;Emr, SD
通讯作者：
Emr, SD
影响因子：
4.8
作者：
Desrivières, S;Cooke, FT;Hall, MN
通讯作者：
Hall, MN
影响因子：
4.8
作者：
Gardner, RG;Shan, H;Hampton, RY
通讯作者：
Hampton, RY