Effects of ovarian fluid on sperm velocity in Arctic charr (Salvelinus alpinus)
Effects of ovarian fluid on sperm velocity in Arctic charr (Salvelinus alpinus)
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卵巢液对北极红点鲑 (Salvelinus alpinus) 精子速度的影响
DOI：
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发表时间：
2005
影响因子：
2.3
通讯作者：
G. Rudolfsen
中科院分区：
文献类型：
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作者：
Davnah Urbach;I. Folstad;G. Rudolfsen
对体外受精鱼类的大量研究提供了卵巢液对精子活力特征（例如向前流动的持续时间、速度或活动精子细胞百分比）的影响的证据。然而，由于女性卵巢液质量的差异，这种影响可能因人而异。此外，不同女性的卵巢液也可能会对每次射精产生不同的影响，从而导致女性选择的神秘性。在这项对北极红点鲑 (Salvelnius alpinus) 的研究中，根据完全平衡杂交设计，在几只雌性的稀释卵巢液中测量了几只雄性的精子速度。这种设计使我们能够估计女性卵巢液对不同男性精子速度影响的差异，并检测男性精子在卵巢液中游动能力的差异。通过计算机辅助精子分析估算精子速度。研究发现，雌性的平均速度存在差异，一些雌性的卵巢液中的平均速度测量值持续较高，而雄性的平均速度则表明，一些雄性的卵巢液中的精子总体速度比其他雄性更快。此外，精子速度的变化取决于雌雄个体的相互作用。我们的结果证明，女性卵巢液对精子速度的影响各不相同，并且根据男性的个体特征，她们的卵巢液可能会刺激精子速度。后一个结果表明了神秘的女性选择的潜在机制。
Numbers of studies in externally fertilizing fish species provide evidence for an effect of ovarian fluid on sperm motility characteristics such as duration of forward mobility, velocity or percent motile sperm cells. Yet, because of variations among females in the quality of their ovarian fluid, such effects might differ between individuals. Additionally, ovarian fluid from different females could also be expected to affect each ejaculate differently, resulting in cryptic female choice. In this study on Artic charr (Salvelnius alpinus), sperm velocity from several males was measured in the diluted ovarian fluid of several females according to a fully balanced crossing design. This design allowed us to estimate variations among females in the effect of their ovarian fluid on the velocity of sperm from different males, and to detect variations among males in the ability of their sperm to swim in ovarian fluid. Sperm velocity was estimated by computer-assisted sperm analysis. Average velocity was found to vary among females, with some females having constantly higher velocity measurements in their ovarian fluid, and among males, indicating that some males had overall faster sperm in ovarian fluid than others. Moreover, variation in sperm velocity was shown to depend on individual female-male interactions. Our results document that females vary in the effect of their ovarian fluid on sperm velocity and that their ovarian fluid may stimulate sperm velocity according to individual characteristics of males. This latter result suggests a potential mechanism for cryptic female choice.