Enrichment, isolation and characterization of pentachlorophenol degrading bacterium Acinetobacter sp. ISTPCP-3 from effluent discharge site
Enrichment, isolation and characterization of pentachlorophenol degrading bacterium Acinetobacter sp. ISTPCP-3 from effluent discharge site
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DOI：
10.1007/s10532-009-9251-5
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发表时间：
2009-02
期刊：
影响因子：
3.6
通讯作者：
Ashwani Sharma;I. Thakur;P. Dureja
中科院分区：
文献类型：
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作者：
Ashwani Sharma;I. Thakur;P. Dureja
从制浆造纸废水排放现场的沉淀芯中分离到3株五氯苯酚降解菌。菌株在添加五氯苯酚作为唯一碳源和能量的矿物盐培养基中不断丰富。本研究筛选出一株降解五氯苯酚能力较高的驯化菌株，并对其进行了特性研究。根据形态、生化测试、16S rDNA序列分析和系统发育特征，这些菌株与不动杆菌属具有最大的相似性。经鉴定，该菌株为不动杆菌属。ISTPCP-3。对该菌株的生理特性和最适生长条件进行了研究。最适生长温度测定结果表明，该菌是一株中温菌。该菌的最适生长温度为30℃，最适初始pH为6.5~7.5，最适pH为7。该菌对五氯苯酚有较强的耐受性，最适降解浓度可达200 mg/L。五氯苯酚浓度的增加对其生物降解率有负面影响，超过250 mg/L的五氯苯酚对其生长有抑制作用。ISTPCP-3能够通过邻位裂解的氧化途径利用PCP，生成2，3，5，6-四氯对苯二酚和2-氯-1，4-苯二酚，用气相色谱-质谱仪(GC-MS)进行鉴定。分离出的细菌所遵循的降解途径不同于以往描述的途径。
Three pentachlorophenol (PCP) degrading bacterial strains were isolated from sediment core of pulp and paper mill effluent discharge site. The strains were continuously enriched in mineral salts medium supplemented with PCP as sole source of carbon and energy. One of the acclimated strains with relatively high PCP degradation capability was selected and characterized in this study. Based on morphology, biochemical tests, 16S rDNA sequence analysis and phylogenetic characteristics, the strains showed greatest similarity withAcinetobacterspp. The strain was identified asAcinetobactersp. ISTPCP-3. The physiological characteristics and optimum growth conditions of the bacterial strain were investigated. The results of optimum growth temperature revealed that it was a mesophile. The optimum growth temperature for the strain was 30°C. The preferential initial pH for the strain was ranging at 6.5–7.5, the optimum pH was 7. The bacterium was able to tolerate and degrade PCP up to a concentration of 200 mg/l. Increase in PCP concentration had a negative effect on biodegradation rate and PCP concentration above 250 mg/l was inhibitory to its growth.Acinetobactersp. ISTPCP-3 was able to utilize PCP through an oxidative route withorthoring-cleavage with the formation of 2,3,5,6-tetrachlorohydroquinone and 2-chloro-1,4-benzenediol, identified using gas chromatograph–mass spectrometric (GC–MS) analysis. The degradation pathway followed by isolated bacterium is different from previously characterized pathway.