Acute cAMP stimulation in Leydig cells: rapid accumulation of a protein similar to that detected in adrenal cortex and corpus luteum.
Acute cAMP stimulation in Leydig cells: rapid accumulation of a protein similar to that detected in adrenal cortex and corpus luteum.
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DOI：
10.3109/07435808609035449
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发表时间：
1986
影响因子：
2.1
通讯作者：
L. Pon;L. Epstein;N. Orme-Johnson
中科院分区：
文献类型：
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作者：
L. Pon;L. Epstein;N. Orme-Johnson
向小鼠Leydig细胞的原代培养物中加入cAMP（作为双丁酰化合物），引起蛋白质i（B）的积累，其对cAMP浓度的依赖性与睾酮合成的增加相同。放线菌酮抑制蛋白质i（B）和睾酮产生的刺激。此外，cAMP还抑制了另一种蛋白质p（B）的合成，p（B）与i（B）的分子量大致相同（28，000道尔顿），但碱性等电点更高。这种行为类似于肾上腺皮质中记录的事件（Krueger，R. J.，和Orme-Johnson，N.R.，J. Biol. Chem.，258，10159-10167，1983）和黄体（Pon，L.A.，和Orme-Johnson，N.R.，J. Biol. Chem.，261，6594-6599，1986）。在第三种产生类固醇的细胞类型中发现这些蛋白质以及引起类固醇合成增加的条件与i（B）产生增加之间的密切相关性进一步表明蛋白质i（B）可能是类固醇激素生物合成的肽激素或cAMP控制中的中介。
Addition of cAMP (as the dibutyryl compound) to a primary culture of mouse Leydig cells caused the accumulation of a protein, i(b), with the same dependence on cAMP concentration as the increase in testosterone synthesis. Stimulation of both protein i(b) and testosterone production were inhibited by cycloheximide. Additionally, cAMP caused repression of synthesis of another protein, p(b), with the same approximate molecular weight (28,000 daltons) as i(b), but more basic isoelectric point. This behavior resembles an event which has been documented in the adrenal cortex (Krueger, R.J., and Orme-Johnson, N.R., J. Biol. Chem., 258, 10159-10167, 1983) and corpus luteum (Pon, L.A., and Orme-Johnson, N.R., J. Biol. Chem., 261, 6594-6599, 1986). The discovery of these proteins in a third steroid-producing cell type and the close correlation between conditions causing increased steroid synthesis and increased i(b) production is further indication that protein i(b) may be an intermediary in peptide hormone or cAMP control of steroid hormone biosynthesis.