Single-Cell Detection and Photostimulation on a Microfluidic Chip Aided with Gold Nanorods
Single-Cell Detection and Photostimulation on a Microfluidic Chip Aided with Gold Nanorods
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金纳米棒辅助的微流控芯片上的单细胞检测和光刺激
DOI：
10.1002/cyto.a.23855
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发表时间：
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通讯作者：
He Hao
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作者：
Zhu Yujie;Xu Hui;Wei Xunbin;He Hao
金纳米棒（GNRs）可以很容易地设计和合成，以响应近红外（NIR）波段的光子。激光照射的光刺激可以通过GNRs介导和增强，从而对细胞引入局部损伤，用于光动力/光热治疗（PDT或PTT）。在这项研究中，我们表明，GNRs染色的细胞可以通过飞秒激光照射在微流体系统上的短闪光同时有效地检测和刺激。在相对高通量的细胞流中，可以激发和检测来自GNR的双光子发光。GNR还介导和增强细胞的瞬时光刺激。在光刺激后，细胞可以分别保持存活、凋亡或坏死。刺激效果强烈依赖于光子密度和刺激持续时间。我们发现细胞存活、凋亡或坏死依赖于GNR染色、激光照射模式和持续时间。因此，我们的系统为高通量细胞刺激和芯片分析提供了简单有效的方法。© 2019 International Society for Advancement of Cytometry
Gold nanorods (GNRs) can be easily designed and synthesized to respond to photons in the near infrared (NIR) band. The photostimulation by laser irradiation can be mediated and enhanced by GNRs to introduce localized damage to cells for photodynamic/photothermal therapy (PDT or PTT). In this study, we show that cells stained with GNRs can be detected and stimulated simultaneously by short flashes of femtosecond‐laser irradiation on a microfluidic system effectively. In the relatively high‐throughput cell flow, the two‐photon luminescence from GNRs can be excited and detected. The GNRs also mediate and enhance the transient photostimulation of the cells. After photostimulation, cells can remain alive, go to apoptosis, or necrosis, respectively. The stimulation effect is strongly dependent on the photon density and stimulation duration. We found the cells remain alive, go to apoptosis or necrosis, dependent on the GNR staining, the laser illumination pattern and duration. Hence, our system provides a simple and effective method for high‐throughput cell stimulation and analysis on chip. © 2019 International Society for Advancement of Cytometry