Cloning and characterization of the chlorophyll biosynthesis gene chlM from Synechocystis PCC 6803 by complementation of a bacteriochlorophyll biosynthesis mutant of Rhodobacter capsulatus.
Cloning and characterization of the chlorophyll biosynthesis gene chlM from Synechocystis PCC 6803 by complementation of a bacteriochlorophyll biosynthesis mutant of Rhodobacter capsulatus.
复制标题
通过与荚膜红细菌的细菌叶绿素生物合成突变体互补，克隆和表征来自集胞藻 PCC 6803 的叶绿素生物合成基因 chlM。
DOI：
10.1007/bf00019561
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发表时间：
1996
影响因子：
5.1
通讯作者：
Bauer,CE
中科院分区：
文献类型：
--
作者：
Smith,CA;Suzuki,JY;Bauer,CE
A bacteriochlorophyllabiosynthesis mutant of the purple photosynthetic bacteriumRhodobacter capsulatuswas functionally complemented with a cosmid genomic library fromSynechocystissp. PCC 6803. The complementedR. capsulatusstrain contains a defined mutation in thebchMgene that codes for Mg-protoporphyrin IX methyltransferase, the enzyme which converts Mg-protoporphyrin IX to Mg-protoporphyrin IX methylester usingS-adenosyl-l-methionine as a cofactor. Since chlorophyll biosynthesis also requires the same methylation reaction, theSynechocystisgenome should similarly code for a Mg-protoporphyrin IX methyltransferase. Sequence analysis of the complementingSynechocystiscosmid indicates that it contains an open reading frame exhibiting 29% sequence identity to BchM. In addition, expression of theSynechocystisgene in theR. capsulatus bchMmutant via the strongR. capsulatus pucpromoter was shown to support nearly wild-type levels of bacteriochlorophyllasynthesis. To our knowledge, theSynechocystissequence thus represents the first chlorophyll biosynthesis gene homolog ofbchM. The complementingSynechocystiscosmid was also shown to code for a gene product that is a member of a highly conserved family of RNA binding proteins, the function of which in cyanobacteria remains undetermined.
