High alcohol intake in female Sardinian alcohol-preferring rats
High alcohol intake in female Sardinian alcohol-preferring rats
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DOI：
10.1016/j.alcohol.2014.01.001
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发表时间：
2014-06-01
期刊：
影响因子：
2.3
通讯作者：
Gessa, Gian Luigi
中科院分区：
文献类型：
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作者：
Loi, Barbara;Colombo, Giancarlo;Gessa, Gian Luigi
撒丁岛酒精偏好（sP）大鼠已被选择性地培育为高酒精偏好和消费。当暴露于标准的家庭笼2瓶“酒精（10%，v/v）vs.水”选择方案并持续进入时，雄性sP大鼠每天消耗约6 g/kg酒精。相反，当暴露于间歇性（每隔一天一次）访问2瓶含有酒精（20%，v/v）和水，分别，雄性sP大鼠显示每日酒精摄入量和酒精中毒和“行为”依赖的迹象显着增加。本研究旨在评估雌性sP大鼠在2瓶选择方案下暴露于（a）10%（v/v）酒精连续进入（CA 10%）、（B）10%（v/v）酒精间歇进入（IA 10%）、（c）20%（v/v）酒精连续进入（CA 20%）、（d）10%（v/v）酒精间歇进入（IA 10%）、（c）20%（v/v）酒精间歇进入（IA 20%）、（d）10%（v/v）酒精间歇进入（IA 20%）、（e）20%（v/v）酒精间歇进入（IA 20%）、（f）20%（v/v）酒精间歇进入（IA 20%）、（e）20%（v/v）酒精间歇进入（IA 20%）、（f）20%（v/v）酒精间歇进入（IA 20%）、（f）20%（v/v/v（d）20%（v/v）酒精间歇性摄入（IA20%）。纳入雄性sP大鼠（暴露于CA 10%和IA 20%条件）进行比较。每日饮酒20次以上，CA 10%和IA 20%雌性大鼠的每日酒精摄入量分别为7.0和9.6g/kg。酒精摄入量大小顺序为IA20% > IA10% = CA20% > CA10%。相反，雄性CA 10%和IA 20%大鼠的每日酒精摄入量平均分别为6.0和8.2 g/kg。雌、雄大鼠酒精摄入量的比较结果为：雌IA20% >雄IA20% >雌CA10% >雄CA10%。另一项实验发现，在饮酒的第一个小时内饮酒，CA 10%和IA 20%大鼠的平均血液酒精水平分别为35-40 mg%和85-100 mg%。这些结果（a）扩展到雌性sP大鼠，先前的数据证明了IA 20%条件显著增加饮酒的能力，并且（B）证明了雌性sP大鼠比雄性sP大鼠消耗更多的酒精。这种性别差异在IA20%条件下更为明显，表明雌性sP大鼠对IA20%条件对饮酒的促进作用高度敏感。这些数据有助于sP大鼠作为过度饮酒模型的表征。(C)2014爱思唯尔公司All rights reserved.
Sardinian alcohol-preferring (sP) rats have been selectively bred for high alcohol preference and consumption. When exposed to the standard, home cage 2-bottle "alcohol (10%, v/v) vs. water" choice regimen with continuous access, male sP rats consume daily approximately 6 g/kg alcohol. Conversely, when exposed to the intermittent (once every other day) access to 2 bottles containing alcohol (20%, v/v) and water, respectively, male sP rats display marked increases in daily alcohol intake and signs of alcohol intoxication and "behavioral" dependence. The present study was designed to assess alcohol intake in female sP rats exposed, under the 2-bottle choice regimen, to (a) 10% (v/v) alcohol with continuous access (CA10%), (b) 10% (v/v) alcohol with intermittent access (IA10%), (c) 20% (v/v) alcohol with continuous access (CA20%), and (d) 20% (v/v) alcohol with intermittent access (IA20%). Male sP rats (exposed to CA10% and IA20% conditions) were included for comparison. Over 20 daily drinking sessions, daily alcohol intake in female CA10% and IA20% rats averaged 7.0 and 9.6 g/kg, respectively. The rank of alcohol intake was IA20% > IA10% = CA20% > CA10%. Conversely, daily alcohol intake in male CA10% and IA20% rats averaged 6.0 and 8.2 g/kg, respectively. Comparison of female and male rats yielded the following rank of alcohol intake: female IA20% > male IA20% > female CA10% > male CA10%. An additional experiment found that alcohol drinking during the first hour of the drinking session produced mean blood alcohol levels of 35-40 mg% and 85-100 mg% in the CA10% and IA20% rats, respectively. These results (a) extend to female sP rats previous data demonstrating the capacity of the IA20% condition to markedly escalate alcohol drinking, and (b) demonstrate that female sP rats consume more alcohol than male sP rats. This sex difference is more evident under the IA20% condition, suggesting that female sP rats are highly sensitive to the promoting effect of the IA20% condition on alcohol drinking. These data contribute to the characterization of sP rats as a model of excessive alcohol consumption. (C) 2014 Elsevier Inc. All rights reserved.