Determination of O:4 antigen-antibody affinity level in O:5 antigen positive and negative variants of Salmonella enterica serovar Typhimurium.
Determination of O:4 antigen-antibody affinity level in O:5 antigen positive and negative variants of Salmonella enterica serovar Typhimurium.
复制标题
确定鼠伤寒沙门氏菌 O:5 抗原阳性和阴性变种的 O:4 抗原-抗体亲和力水平。
DOI：
10.1093/femsle/fnx062
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发表时间：
2017
影响因子：
2.1
通讯作者：
Eguchi M.
中科院分区：
文献类型：
--
作者：
Nakai Y;Ito A;Ogawa Y;Aribam SD;Elsheimer-Matulova M;Shiraiwa K;Kisaka SMB;Hikono H;Nishikawa S;Akiba M;Kawahara K;Shimoji Y;Eguchi M.
鼠伤寒沙门氏菌 (S.Typhimurium) 有两种血清学变异：一种表达 O:5 抗原 (1,4,5,12:i:1,2)，另一种缺乏 O:5 抗原 (1,4,12:i:1,2)。对于血清分型，鼠伤寒沙门氏菌通过诊断 O:4 抗原血清进行凝集。本研究旨在比较鼠伤寒沙门氏菌χ3306 O:5阳性菌株和鼠伤寒沙门氏菌χ3306 O:5阴性菌株中O:4抗原的抗原抗体亲和力。 The affinity of O:4 antigen with O:4 antigen serum was found to be stronger in the O:5-negative strains compared to O:5-positive strains.接下来，我们研究了 O:4 抗原与 S 野生株中 O:4 抗原血清的抗原抗体亲和力。鼠伤寒沙门氏菌，其显示出与鼠伤寒沙门氏菌χ3306 O:5-阳性和阴性菌株相同的亲和力趋势。这项研究表明，O:5 抗原的存在或不存在会导致不同野生 S 菌株的 O:4 凝集反应存在差异。鼠伤寒杆菌。
Salmonella entericaserovar Typhimurium (S.Typhimurium) has two serological variants: one that expresses the O:5 antigen (1,4,5,12:i:1,2) and one that lacks O:5 antigen (1,4,12:i:1,2). For serotyping,S.Typhimurium is agglutinated by diagnostic O:4 antigen serum. This study was carried out to compare the antigen-antibody affinity of O:4 antigen inS.Typhimurium χ3306 O:5-positive andS.Typhimurium χ3306 O:5-negative strains. The affinity of O:4 antigen with O:4 antigen serum was found to be stronger in the O:5-negative strains compared to O:5-positive strains. Next, we investigated the antigen-antibody affinity of O:4 antigen with O:4 antigen serum in field strains ofS. Typhimurium, which showed the same tendency in affinity as seen withS.Typhimurium χ3306 O:5-positive and negative strains. This study suggests that the presence or absence of O:5 antigen causes differences in O:4 agglutination reactions with different field strains ofS. Typhimurium.