The Effect of Single Versus Group μCT on the Detection of Trabecular and Cortical Disease Phenotypes in Mouse Bones.
The Effect of Single Versus Group μCT on the Detection of Trabecular and Cortical Disease Phenotypes in Mouse Bones.
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DOI：
10.1002/jbm4.10473
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发表时间：
2021-04
期刊：
影响因子：
3.8
通讯作者：
Wallace JM
中科院分区：
文献类型：
--
作者：
Kohler R;Tastad CA;Stacy AJ;Swallow EA;Metzger CE;Allen MR;Wallace JM
微型计算机断层扫描是骨相关临床前研究的重要评估工具，特别是在小鼠模型中。为了加快扫描过程，研究人员经常同时对多块骨骼进行成像；然而，尚不清楚这是否会影响扫描质量，并改变检测试验组之间差异的能力。本研究的目的是通过分组扫描两种已知骨骼缺陷的小鼠模型：成骨不全的Col1a2 G610C/+模型和腺嘌呤诱导的慢性肾脏疾病模型，评估多骨扫描在检测疾病引起的骨微结构和骨密度变化方面的效果。成年男性股骨分别在Bruker Skyscan1172和1176上进行单独扫描和随机分组扫描，每组3个和8个，然后评估标准的骨小梁和皮质骨测量。尽管扫描方法改变了原始值，小梁微结构值比皮质特性受到的影响更大，但在两组和单独扫描中仍可检测到疾病表型。然而，与单独扫描相比，组扫描对骨小梁和皮质骨的组织密度都有显著影响。当整体骨表型较大时，研究人员可以在一次μCT扫描中使用小分组，以加快临床前分析，以降低成本和加快发现速度；但是，应该始终报告扫描的细节(单扫描或组扫描)。©2021作者。JBMR Plus由威利期刊有限责任公司代表美国骨骼和矿物研究学会出版。
Micro‐computed tomography is a critical assessment tool for bone‐related preclinical research, especially in murine models. To expedite the scanning process, researchers often image multiple bones simultaneously; however, it is unknown if this impacts scan quality and alters the ability to detect differences between experimental groups. The purpose of this study was to assess the effect of multibone scanning on detecting disease‐induced changes in bone microarchitecture and mineral density by group scanning two murine models with known skeletal defects: the Col1a2 G610C/+ model of osteogenesis imperfecta and an adenine‐induced model of chronic kidney disease. Adult male femurs were scanned individually and in random groups of three and eight in a Bruker Skyscan 1172 and 1176, respectively, then assessed for standard trabecular and cortical bone measures. Although scanning methodology altered raw values, with trabecular microarchitecture values more affected than cortical properties, a disease phenotype was still detectable in both group and solo scans. However, tissue mineral density in both trabecular and cortical bone was significantly impacted by group versus solo scanning. Researchers may be able to use small groupings in a single μCT scan to expedite preclinical analyses when the overall bone phenotype is large to decrease costs and increase speed of discoveries; however the details of scanning (single or group) should always be reported. © 2021 The Authors. JBMR Plus published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research.