CIRCADIAN-RHYTHM IN MEMBRANE CONDUCTANCE EXPRESSED IN ISOLATED NEURONS
CIRCADIAN-RHYTHM IN MEMBRANE CONDUCTANCE EXPRESSED IN ISOLATED NEURONS
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DOI：
10.1126/science.8421785
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发表时间：
1993-01-08
期刊：
影响因子：
56.9
通讯作者：
BLOCK, GD
中科院分区：
文献类型：
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作者：
MICHEL, S;GEUSZ, ME;BLOCK, GD
虽然孤立的神经元可以产生节律性活动，但尚未显示它们产生具有昼夜节律范围内的周期（接近24小时）的节律。软体动物Bulla gouldiana的眼睛在视神经脉冲中表达昼夜节律，所述视神经脉冲由称为基底视网膜神经元（BRN）的电耦合细胞产生。BRN的膜电位的每日波动似乎是由膜电导的节律驱动的。孤立的BRN表现出自发的电导变化类似于在完整的视网膜中观察到的。膜电导高，在主观的深夜，并在至少两个昼夜节律周期的文化，预计黎明附近下降约两倍。在孤立的BRN中持续的每日电导变化表明单个神经元可以作为昼夜节律起搏器。
Although isolated neurons can generate rhythmic activity, they have not yet been shown to generate rhythms with a period in the circadian range (near 24 hours). The eye of the mollusk Bulla gouldiana expresses a circadian rhythm in optic nerve impulses that is generated by electrically coupled cells known as basal retinal neurons (BRNs). Daily fluctuations in the membrane potential of the BRNs appear to be driven by a rhythm in membrane conductance. Isolated BRNs exhibited spontaneous conductance changes similar to those observed in the intact retina. Membrane conductance was high in the late subjective night and decreased approximately twofold near projected dawn during at least two circadian cycles in culture. The persistence of daily conductance changes in isolated BRNs indicates that individual neurons can function as circadian pacemakers.