Multidimensional drug profiling by automated microscopy
Multidimensional drug profiling by automated microscopy
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DOI：
10.1126/science.1100709
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发表时间：
2004-11-12
期刊：
影响因子：
56.9
通讯作者：
Altschuler, SJ
中科院分区：
文献类型：
--
作者：
Perlman, ZE;Slack, MD;Altschuler, SJ
We present a method for high-throughput cytological profiling by microscopy. Our system provides quantitative multidimensional measures of individual cell states over wide ranges of perturbations. We profile dose-dependent phenotypic effects of drugs in human cell culture with a titration-invariant similarity score (TISS). This method successfully categorized blinded drugs and suggested targets for drugs of uncertain mechanism. Multivariate single-cell analysis is a starting point for identifying relationships among drug effects at a systems level and a step toward phenotypic profiting at the single-cell level. Our methods will be useful for discovering the mechanism and predicting the toxicity of new drugs.
