iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure.
iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure.
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基于 iTRAQ 的 IVF 失败后实现救援 ICSI 妊娠的正常精子男性的精子蛋白质组分析
DOI：
10.1186/s12014-018-9203-3
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发表时间：
2018
影响因子：
3.8
通讯作者：
Liu F
中科院分区：
文献类型：
--
作者：
Liu X;Liu G;Liu J;Zhu P;Wang J;Wang Y;Wang W;Li N;Wang X;Zhang C;Shen X;Liu F
在辅助生殖中，由于精子缺乏与正常透明带结合而导致体外受精(IVF)不明原因失败，并通过抢救性卵胞浆内单精子注射(R-ICSI)获得妊娠的正常精子者，其精子不育分子仍不清楚。还需要更多的工作来探索这种男性不育的机制。收集IVF妊娠的正常精子症男性和常规IVF失败后R-ICSI妊娠的正常精子症男性。以iTRAQ为基础的蛋白质组学方法揭示了IVF和R-ICSI妊娠男性之间新的不孕原因。为了验证蛋白质组数据的可信度，对单个样本进行了蛋白质印迹和免疫荧光分析。此外，通过检测自发性顶体反应来评价精子质量。与IVF妊娠组相比，R-ICSI妊娠组有56个精子蛋白表达差异。生物信息学分析(Panther、David、PubMed和STRING)表明，这些改变的精子蛋白参与了多种分子功能：生殖、染色体组织和精子-卵母细胞相互作用。此外，利用单个样本的蛋白质印迹和免疫荧光验证了蛋白质组数据的可信度，这与我们的蛋白质组数据一致。此外，在R-ICSI妊娠组中发现自发性顶体反应率增加。精子蛋白的改变和自发性顶体反应率的增加可能是R-ICSI妊娠患者中原因不明的男性不育的原因。目前的蛋白质组学结果将有助于更好地理解这些在试管受精失败后实现R-ICSI妊娠的正常精子症男性背后的不明原因的不育机制。这篇文章的在线版本(10.1186/s12014-0189203-3)包含补充材料，授权用户可以使用。
In the assisted reproduction, the infertile molecules of spermatozoa from normozoospermic men who underwent the unexplained failure of in vitro fertilization (IVF) due to the lack of sperm binding to the normal zona pellucida, and then achieved pregnancy with the rescue intracytoplasmic sperm injection (R-ICSI) remain unclear. More works are still necessary to explore this male infertile mechanism. Normozoospermicmen with the IVF pregnancy and normozoospermic men with the R-ICSI pregnancy after the conventional IVF failure were collected. iTRAQ-based proteomic approach were performed to reveal the new infertile causes between the IVF pregnancy men and the R-ICSI pregnancy men. To validate the confidence of proteome data, the individual samples were analyzed by western blot and immunofluorescence. Further, the spontaneous acrosome reactions were measured to evaluate the sperm quality. Compared with IVF pregnancy group, 56 sperm proteins were differentially expressed in the R-ICSI pregnancy group. Bioinformatic analyses (PANTHER, DAVID, PubMed and STRING) indicated these altered sperm proteins were involved in various molecular functions: reproduction, chromosome organization, and sperm-oocyte interaction. Moreover, the confidence of proteome data was confirmed by western blot and immunofluorescence using the individual samples, which were consistent with our proteomic data. Additionally, an increased rate of the spontaneous acrosome reaction rate was found in the R-ICSI pregnancy group. The sealtered sperm proteins and the increased spontaneous acrosome reaction rate might account for this unexplained male infertility in the R-ICSI pregnancy patients. The present proteomic results will throw light on the better understanding of the unexplained infertile mechanisms underlying these normozoospermic man who achieved R-ICSI pregnancy after IVF failure. The online version of this article (10.1186/s12014-018-9203-3) contains supplementary material, which is available to authorized users.
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影响因子：
4.6
作者：
Sun W;Xing B;Guo L;Liu Z;Mu J;Sun L;Wei H;Zhao X;Qian X;Jiang Y;He F
通讯作者：
He F
影响因子：
2.9
作者：
GOTHAM, SM;FRYER, PJ;PATERSON, WR
通讯作者：
PATERSON, WR
影响因子：
2.9
作者：
Tanphaichitr N;Kongmanas K;Kruevaisayawan H;Saewu A;Sugeng C;Fernandes J;Souda P;Angel JB;Faull KF;Aitken RJ;Whitelegge J;Hardy D;Berger T;Baker M
通讯作者：
Baker M
影响因子：
6.7
作者：
Frapsauce, Cynthia;Pionneau, Cedric;Mandelbaum, Jacqueline
通讯作者：
Mandelbaum, Jacqueline
影响因子：
6.7
作者：
Liu, DY;Baker, HWG
通讯作者：
Baker, HWG