Soluble suppressor supernatants elaborated by concanavalin A-activated human mononuclear cells. I. Characterization of a soluble suppressor T cell proliferation.
Soluble suppressor supernatants elaborated by concanavalin A-activated human mononuclear cells. I. Characterization of a soluble suppressor T cell proliferation.
复制标题
由伴刀豆球蛋白 A 激活的人单核细胞精制而成的可溶性抑制物上清液。
DOI：
10.4049/jimmunol.126.3.1185
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发表时间：
1981
影响因子：
4.4
通讯作者：
T. Waldmann
中科院分区：
文献类型：
--
作者：
W. Greene;T. Fleisher;T. Waldmann
当被促有丝分裂凝集素伴刀豆球蛋白A激活时，人外周血单核细胞的一个亚群分化成负调节细胞和体液免疫反应的有效抑制细胞。除了抑制性细胞间相互作用外，这些调节细胞还产生含有至少2种不同抑制因子的可溶性免疫抑制因子上清液（SISS）。这些因子之一SISS-T抑制有丝分裂原和抗原刺激的T细胞增殖，而另一个SISS-B抑制B细胞免疫球蛋白产生。后一种抑制剂的特性见配套文件。可溶性T细胞增殖抑制因子（SISS-T）的特性包括：1）分子量为1000 - 10000 μ g/L; 30 - 45，000，2）通过非细胞毒性机制抑制，3）在56 ℃不稳定，4）在单糖N-乙酰基-D-葡糖胺存在下丧失活性并保留在N-乙酰基-D-葡糖胺亲和柱上，5）与凝集素小麦胚芽凝集素和双孢蘑菇凝集素识别的相同表面糖蛋白受体结合，其对有丝分裂原或抗原诱导的淋巴细胞增殖产生类似的抑制，6）通过用500和2000 R但不是6000 R照射的细胞进行的加工，7）最少需要24小时的凝集素刺激以产生，和8）通过贴壁细胞或可选地需要贴壁细胞参与的细胞协作进行加工。这些数据表明，人抑制细胞能够通过产生与确定的表面糖蛋白或糖脂受体相互作用的可溶性糖特异性因子来调节T细胞功能。这些被抑制性内源凝集素识别的相同受体也被选择的外源性非促有丝分裂凝集素激活，所述外源性非促有丝分裂凝集素产生类似的T细胞代谢抑制。
When activated with the mitogenic lectin, concanavalin A, a subset of human peripheral blood mononuclear cells differentiate into potent suppressor cells that negatively modulate both cellular and humoral immune reactions. In addition to inhibitory cell-cell interactions, these regulatory cells elaborate a soluble immune suppressor supernatant (SISS) containing at lest 2 distinct suppressor factors. One of these factors, SISS-T, inhibits mitogen- and antigen-stimulated T cell proliferation, whereas the other, SISS-B, inhibits B cell immunoglobulin production. Characteristics of the latter inhibitor are reported in the companion paper. Properties of the soluble suppressor of T cell proliferation (SISS-T) include: 1) a m.w. of 30 to 45,000, 2) inhibition by a noncytotoxic mechanism, 3) instability at 56 degrees C, 4) loss of activity in the presence of the monosaccharide N-acetyl-D-glucosamine and retention on N-acetyl-D-glucosamine affinity columns, 5) binding to the same surface glycoprotein receptors recognized by the lectins wheat germ agglutinin and Agaricus bisporus lectin, which produce similar inhibition of mitogen- or antigen-induced lymphocyte proliferation, 6) elaboration by cells irradiated with 500 and 2000 R but not 6000 R, 7) a minimum requirement for 24 hr of lectin stimulation for production, and 8) elaboration by adherent cells or alternatively cellular collaboration requiring the participation of adherent cells. These data indicate that human suppressor cells are capable of modulating T cell function via the production of a soluble saccharide-specific factor(s) that interacts with defined surface glycoprotein or glycolipid receptors. These same receptors recognized by the suppressive endogenous lectin are also activated by selected exogenous nonmitogenic lectins that produce similar inhibition of T cell metabolism.