Strict tropism for CD71+/CD234+ human reticulocytes limits the zoonotic potential of Plasmodium cynomolgi
Strict tropism for CD71+/CD234+ human reticulocytes limits the zoonotic potential of Plasmodium cynomolgi
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DOI：
10.1182/blood-2017-02-764787
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发表时间：
2017-09-14
期刊：
影响因子：
20.3
通讯作者：
Russell, Bruce
中科院分区：
文献类型：
--
作者：
Kosaisavee, Varakorn;Suwanarusk, Rossarin;Russell, Bruce
东南亚猕猴的两种疟疾寄生虫，诺氏疟原虫和食蟹猴疟原虫，在实验上可以感染人类。在这两种疟疾都很常见的马来西亚，人畜共患诺氏疟疾最近已成为主导疾病，整个地区都有病例记录。相比之下，迄今为止，在人类中只发现了一例自然获得的食蟹猴丙酸杆菌。在这项研究中，我们发现食蟹猴 P 食蟹猴在体外不加区别地侵入猴红细胞，但在人类中，它们仅限于表达转铁蛋白受体 1（Trf1 或 CD71）和达菲抗原/趋化因子受体（DARC 或 CD234）的网织红细胞。这可能导致可检测到的人畜共患食蟹猴疟疾的缺乏。我们进一步描述了食蟹猴疟原虫感染的人网织红细胞入侵后的形态学和流变学变化，这些变化与间日疟原虫所观察到的惊人相似。这些观察结果强调了食蟹猴作为模型在开发针对间日疟疾的血液阶段疫苗中的价值。
Two malaria parasites of Southeast Asian macaques, Plasmodium knowlesi and P cynomolgi, can infect humans experimentally. In Malaysia, where both species are common, zoonotic knowlesi malaria has recently become dominant, and cases are recorded throughout the region. By contrast, to date, only a single case of naturally acquired P cynomolgi has been found in humans. In this study, we show that whereas P cynomolgimerozoites invade monkey red blood cells indiscriminately in vitro, inhumans, they are restricted to reticulocytes expressing both transferrin receptor 1 (Trf1 or CD71) and the Duffy antigen/chemokine receptor (DARC or CD234). This likely contributes to the paucity of detectable zoonotic cynomolgi malaria. We further describe postinvasion morphologic and rheologic alterations in P cynomolgi-infected human reticulocytes that are strikingly similar to those observed for P vivax. These observations stress the value of P cynomolgi as a model in the development of blood stage vaccines against vivax malaria.