Longitudinal evaluation of aflatoxin exposure in two cohorts in south-western Uganda
Longitudinal evaluation of aflatoxin exposure in two cohorts in south-western Uganda
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DOI：
10.1080/19440049.2015.1048749
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发表时间：
2015-08-03
影响因子：
2.9
通讯作者：
Wang, Jia-Sheng
中科院分区：
文献类型：
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作者：
Kang, Min-Su;Nkurunziza, Peter;Wang, Jia-Sheng
黄曲霉毒素（AF）是一组霉菌毒素。 AF 暴露会对人类（尤其是发展中国家）造成急性和慢性不良健康影响，例如黄曲霉中毒和肝细胞癌。在这项研究中，使用来自乌干达西南部两项队列研究的人类免疫缺陷病毒 (HIV) 血清阴性参与者的存档血清样本评估了房颤暴露情况。通过 HPLC 荧光测定来自一般人群队列 (GPC) 的总共 713 份血清样品中的 AFB(1)-赖氨酸 (AFB-Lys) 加合物水平，涵盖 1989 年至 2010 年间的 8 个时间段。总体而言，90% (642/713) 的样品呈 AFB-Lys 阳性，中位水平为 1.58pg mg(-1) 白蛋白（范围 = 0.40-168pg mg(-1)白蛋白)。还测量了 Rakai 社区队列研究 (RCCS) 1999 年至 2003 年间四个时间段的总共 374 个血清样本中的 AFB-Lys 加合物水平。平均检出率为 92.5% (346/374)，中位水平为 1.18pg mg(-1) 白蛋白（范围 = 0.40-122.5pg mg(-1) 白蛋白）。在 GPC 研究中，年龄、性别和教育水平等人口统计参数与血清 AFB-Lys 加合物水平之间没有统计学上的显着差异。在 RCCS 研究中，使用广义估计方程的纵向分析揭示了加合物水平与居住区 (p=0.05) 和职业 (p=0.02) 之间的显着差异。这项研究表明，乌干达西南部两个人群的房颤暴露情况是持续存在的，并且随着时间的推移没有发生显着变化。一项研究（而非另一项研究）的数据表明，农业工人和农村居民比非农业工人和非农村居民有更多的房颤暴露机会。这些结果表明需要进一步研究房颤引起的人类不良健康影响，特别是该地区的主要疾病。
Aflatoxins (AF) are a group of mycotoxins. AF exposure causes acute and chronic adverse health effects such as aflatoxicosis and hepatocellular carcinoma in human populations, especially in the developing world. In this study, AF exposure was evaluated using archived serum samples from human immunodeficiency virus (HIV)-seronegative participants from two cohort studies in south-western Uganda. AFB(1)-lysine (AFB-Lys) adduct levels were determined via HPLC fluorescence in a total of 713 serum samples from the General Population Cohort (GPC), covering eight time periods between 1989 and 2010. Overall, 90% (642/713) of the samples were positive for AFB-Lys and the median level was 1.58pg mg(-1) albumin (range = 0.40-168pg mg(-1) albumin). AFB-Lys adduct levels were also measured in a total of 374 serum samples from the Rakai Community Cohort Study (RCCS), across four time periods between 1999 and 2003. The averaged detection rate was 92.5% (346/374) and the median level was 1.18pg mg(-1) albumin (range = 0.40-122.5pg mg(-1) albumin). In the GPC study there were no statistically significant differences between demographic parameters, such as age, sex and level of education, and levels of serum AFB-Lys adduct. In the RCCS study, longitudinal analysis using generalised estimating equations revealed significant differences between the adduct levels and residential areas (p=0.05) and occupations (p=0.02). This study indicates that AF exposure in people in two populations in south-western Uganda is persistent and has not significantly changed over time. Data from one study, but not the other, indicated that agriculture workers and rural area residents had more AF exposure than those non-agricultural workers and non-rural area residents. These results suggest the need for further study of AF-induced human adverse health effects, especially the predominant diseases in the region.